CC-1919 fla10-1 mt-
$30.00
From David Luck, Rockefeller University, July 1985
Phenotype: impaired motility
The fla10 mutant cannot regenerate flagella at 32 degrees. The original fla10 allele (fla10-1) is a C to A transversion that alters amino acid 329 in the motor domain of the kinesin-homologous KHP1 protein. Other alleles at this locus include the null murant fla10-2 mutant (CC-4180), and fla10-14 (CC-1395).
Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85
Walther Z, Vashishtha M, Hall JL (1994) The Chlamydomonas FLA10 gene encodes a novel kinesin-homologous protein. J Cell Biol 126:175-188
Kozminski KG, Beech PL, Rosenbaum JL (1995) The Chlamydomonas kinesin-like protein FLA10 is involved in motility associated with the flagellar membrane. J Cell Biol 131:1517-1527
Piperno G, Mead K, Henderson S (1996) Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1(FLA10) to reach the distal part of flagella in Chlamydomonas. J Cell Biol 133:371-379
Vashishtha M, Walther Z, Hall JL (1996) The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles. J Cell Sci 109:541-549
Cole DG, Diener DR, Himelblau AL, Beech PL, Fuster JC, Rosenbaum JL (1998) Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons. J Cell Biol 141:993-1008
Matsuura K, Lefebvre PA, Kamiya R, Hirono M (2002) Kinesin-II is not essential for mitosis and cell growth in Chlamydomonas. Cell Motil Cytoskeleton 52:195-201
Mueller J, Perrone CA, Bower R, Cole DG, Porter ME (2005) The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport. Mol Biol Cell 16:1341-1354
Miller MS, Esparza JM, Lippa AM, Lux FG 3rd, Cole DG, Dutcher SK (2005) Mutant kinesin-2 motor subunits increase chromosome loss. Mol Biol Cell 16:3810-3820