CC-1995 mat3-2 spr-u-1-27-3 mt+
$30.00
N.W. Gillham, Duke University, May 1986
Phenotype: abnormal cell division; antibiotic resistant (spectinomycin)
This is Gillham’s original mat3-2 isolate, designated M3-5-2-5. Originally thought to have modified transmission of the chloroplast genome, the mat3 mutants have been shown by Umen and Goodenough to have lesions in a retinoblastoma protein homolog involved in cell division. The altered plastid gene transmission is a secondary consequence of abnormally small cell size.
This mutation may become suppressed, and outcrossing to wild type may be necessary to recover the original phenotype.
Gillham NW, Boynton JE, Johnson AM, Burkhart BD (1987) Mating type linked mutations which disrupt the uniparental transmission of chloroplast genes in chlamydomonas. Genetics 115:677-684
Armbrust EV, Ibrahim A, Goodenough UW (1995) A mating type-linked mutation that disrupts the uniparental inheritance of chloroplast DNA also disrupts cell-size control in Chlamydomonas. Mol Biol Cell 6:1807-1818
Umen JG, Goodenough UW (2001) Control of cell division by a retinoblastoma protein homolog in Chlamydomonas. Genes Dev 15:1652-1661