* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
CC-2061 cr1 mt-
$30.00
Chlamydomonas Genetics Center, Duke University, 1986
Phenotype: cold sensitive on minimal medium
From CC-125 x CC-40 cr1 mt- (no longer in the collection)
The cr1 mutant in its original form is deficient in chloroplast ribosomes but accumulates 54S ribosomal subunits. It is a leaky acetate-requirer and is cold sensitive. With time, nearly all cr1 cultures acquire second-site mutations that reduce the accumulation of ribosomal subunit material. The ac20 mutation (see CC-43) is the best characterized of these; we don’t know if all the secondary mutations are ac20 alleles or if some are at different loci. It’s been a long time since this particular strain was tested for 54S accumulation.
Please see CC-41 for more information on cr1.