CC-2487 dd-m-200

$30.00

From Gianni Piperno, Rockefeller University, June 1990

Phenotype: impaired motility

This mutant was isolated by methyl methanesulfonate mutagenesis of cells grown in synchronous cultures (12:12 light-dark), at a time approximately 3 to 4 hours into the dark cycle. Cells resemble wild type at permissive temperature (20 degrees) but when shifted to 32 degrees, the flagella beat in an rapid, uncoordinated manner. Wild-type function is recovered with a shift to permissive temperature, and does not require reassembly.


Huang B, Rifkin MR, Luck DJ (1977) Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii. J Cell Biol 72:67-85