CC-5448 ∆COP5-E6 mt+ [PH103]


From Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, December 2018

This is a COP5 disruption strain, generated with CRISPR/Cas9.

Background strain                 CC-125 mt+
Nuclease                                   (Sp)Cas9 as ribonucleoprotein (RNP)
Marker                                       pAPHVIII (pIS105)
Target gene                              COP5, Cre02.g074150
Target sequence                     GCAGCACCTCCAGACTGACGCGG (Exon 6)


Overview of all CRISPR/Cas9 strains from the Hegemann lab
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Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518