CC-5448 ∆COP5-E6 mt+ [PH103]
$20.00
From Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin, December 2018
This is a COP5 disruption strain, generated with CRISPR/Cas9.
Background strain CC-125 mt+
Nuclease (Sp)Cas9 as ribonucleoprotein (RNP)
Marker pAPHVIII (pIS105)
Target gene COP5, Cre02.g074150
Target sequence GCAGCACCTCCAGACTGACGCGG (Exon 6)
Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de
Greiner A, Kelterborn S, Evers H, Kreimer G, Sizova I, Hegemann P (2017) Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29:2498-2518