CC-5603 amc9; NUO5-FLAG mt- [transformant #18]


From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5-FLAG; APHVIII

This is the amc9 strain complemented with the wild-type NUO5 gene with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the wild-type NUO5 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

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