pALM30-Sp

$30.00

Source: Olivier Vallon, Institut de Biologie Physico-Chimique, Paris, November 2011

Used to modify Chlamydomonas genomic BACs to Sp resistance

Insert: CrSpAadA (AR promoter, RBCS2 3′-UTR)

Origin: pCVD442 with IS1 and CmR removed (NdeI AsiSI) and replaced by bla, then inserting 3 kbp of pBeloBAC11-Nit8 (AscI AgeI), then inserting (FseI) the CrEcAadA marker from pALM33

Vector: R6K

Host strain: S17-1 λpir

Ap Sp resistant

 

Sequence (PDF)

Sequence (.txt)Map