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From Irina Sizova, Peter Hegemann lab, Humboldt University-Berlin October 2017
Streptomyces rimosus aminoglycoside-5 ́- phosphotransferase aphVIII selection marker optimized to C. reinhardtii codon bias, controlled by the HSP70A/RBCS2 tandem promoter and RBCS2 3’ UTR.
Selection in C. reinhardtii: paromomycin (10-40 μg/ml)
Host strain: DH10b
Selection in E. coli: ampicillin (100 µg/ml)
Link to sequence and map (GenBank, updated 09/2019)
Sequence (.docx file)
Overview of all CRISPR/Cas9 plasmids from the Hegemann lab
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de