pCrU6.1-SpCas9 cloning (pPH195)


From Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin October 2017

Cloning vector for Streptococcus pyogenes Cas9 (SpCas9) single guide RNA transcription, controlled by the U6snRNA promoter #1.

Host strain: DH10b
Selection in E. coli: ampicillin
Link to sequence and map
Sequence (.docx file)

Overview of all CRISPR/Cas9 plasmids from the Hegemann lab

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