pHS-SpCas9 (pPH185)


From Simon Kelterborn, Peter Hegemann lab, Humboldt University-Berlin October 2017

Expression of Streptococcus pyogenes (Sp)Cas9 optimized to C. reinhardtii codon bias, controlled by the HSP70A heat-shock promoter.

Host strain: XL1-Blue
Selection in E.coli: ampicillin
Link to sequence and map
Sequence (.docx file)

Overview of all CRISPR/Cas9 plasmids from the Hegemann lab

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