* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.

pPO3

Name: pPO3
SKU: P-1091 (amp)
Availability: InStock

$30.00

From Saul Purton, University College London, August 2023

Vector: pBluescript
Host strain: E. coli DH5α
Insert: The overall size of the plasmid is 9356 bp with the 2059 bp ptxD cassette flanked by homology arms of 2850 bp and 1523 bp (see plasmid map).
Selection: ampicillin resistant

A synthetic ptxD cassette was designed to serve as a selectable marker for chloroplast transformation, allowing selection on media containing phosphite as the P source. The cassette was optimised for expression in the chloroplast and cloned into plasmid pBa3-AX carrying a section of the chloroplast genome encompassing psaA-3, trnL and the 5’ region of ccsA. The ptxD cassette was inserted into a neutral intergenic locus using unique MluI and EcoRI sites between psaA-3 and trnL.

Changko S, Rajakumar PD, Young REB, Purton S. The phosphite oxidoreductase gene, ptxD as a bio-contained chloroplast marker and crop-protection tool for algal biotechnology using Chlamydomonas. Appl Microbiol Biotechnol. 2020 Jan;104(2):675-686. doi: 10.1007/s00253-019-10258-7. Epub 2019 Dec 2. PMID: 31788712; PMCID: PMC6943410.

pPO3

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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