pWUCA1

$30.00

From Rosanna Young, Saul Purton lab, University College London, UK, March 2017

pWUCA1 can be used to introduce the trnWUCA gene downstream of psaA exon 3 in the C. reinhardtii chloroplast genome using spectinomycin selection. trnWUCA is a synthetic tRNA that allows the readthrough of internal UGA codons.

100 μg/ml ampicillin in E. coli. 100 μg/ml spectinomycin for selection of aadA cassette in C. reinhardtii.

Sequence


Young RE & Purton S (2016) Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J. 14:251-60