* The listed price of $30.00 for cultures and plasmids is for academic and non-profit users. Commercial and industrial users are charged $300.00 for each strain or plasmid and twice the listed price for media components. BACs and fosmids are $50.00 for academic and non-profit users and $500 for commercial/industry. For the Jonikas CLiP mutants, the price is $100.00 each for academic and non-profit users and commercial and industrial users are charged $500.00.
pWUCA1
$30.00
From Rosanna Young, Saul Purton lab, University College London, UK, March 2017
pWUCA1 can be used to introduce the trnWUCA gene downstream of psaA exon 3 in the C. reinhardtii chloroplast genome using spectinomycin selection. trnWUCA is a synthetic tRNA that allows the readthrough of internal UGA codons.
100 μg/ml ampicillin in E. coli. 100 μg/ml spectinomycin for selection of aadA cassette in C. reinhardtii.
Young RE & Purton S (2016) Codon reassignment to facilitate genetic engineering and biocontainment in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J. 14:251-60