CLiP Strains
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) NDA1 (Cre16.g691552), chromosome_16 base 7727122 (genome v5.5), intron – position has 95% probability of being correct
2) NDA1 (Cre16.g691552), chromosome_16 base 7727183 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre04.g229163, chromosome_4 base 3709705 (genome v5.5), intron – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre14.g625550 & Cre14.g625600, chromosome_14 base 2546301 (genome v5.5), 3’UTR|outside_mRNA & 3’UTR – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) AAT2 (Cre06.g284700), chromosome_6 base 5486988 (genome v5.5), 3’UTR – position has 95% probability of being correct
2) AAT2 (Cre06.g284700), chromosome_6 base 5487095 (genome v5.5), 3’UTR – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre09.g392252, chromosome_9 base 2317049 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre09.g392252, chromosome_9 base 2317049 (genome v5.5), CDS – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) intergenic, chromosome_8 base 2134278 (genome v5.5) – WARNING position has 73% probability of being correct
2) Cre12.g513350, chromosome_12 base 3540974 (genome v5.5), intron – WARNING position has 58% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre06.g275950, chromosome_6 base 3197409 (genome v5.5), intron – position has 95% probability of being correct
2) Cre06.g275950, chromosome_6 base 3197410 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) FAP290 (Cre06.g306750), chromosome_6 base 8376541 (genome v5.5), CDS – position has 95% probability of being correct
2) FAP290 (Cre06.g306750), chromosome_6 base 8376546 (genome v5.5), CDS – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre03.g155700, chromosome_3 base 1961418 (genome v5.5), intron – position has 95% probability of being correct
2) Cre03.g155700, chromosome_3 base 1961467 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre07.g315550, chromosome_7 base 467486 (genome v5.5), intron – position has 95% probability of being correct
2) Cre07.g315550, chromosome_7 base 467521 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:
1) Cre06.g282700, chromosome_6 base 5279963 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre06.g282700, chromosome_6 base 5279965 (genome v5.5), CDS – position has 95% probability of being correct
3) Cre11.g483100, chromosome_11 base 3763182 (genome v5.5), 3’UTR – position has 95% probability of being correct
4) Cre11.g483100, chromosome_11 base 3763186 (genome v5.5), 3’UTR – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre04.g220850, chromosome_4 base 2353701 (genome v5.5), CDS/intron – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre06.g278198, chromosome_6 base 3995659 (genome v5.5), intron – position has 95% probability of being correct
2) Cre06.g278198, chromosome_6 base 3995698 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) GAK1 (Cre03.g185300), chromosome_3 base 5452321 (genome v5.5), intron – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre04.g227800, chromosome_4 base 3465200 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre04.g227800, chromosome_4 base 3465213 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) FXL1 (Cre13.g586650), chromosome_13 base 3330837 (genome v5.5), intron – position has 95% probability of being correct
2) FXL1 (Cre13.g586650), chromosome_13 base 3330841 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre11.g480300, chromosome_11 base 3335579 (genome v5.5), intron – position has 95% probability of being correct
2) Cre11.g480300, chromosome_11 base 3335579 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) SMM35 (Cre10.g422700), chromosome_10 base 726630 (genome v5.5), CDS – position has 95% probability of being correct
2) SMM35 (Cre10.g422700), chromosome_10 base 726634 (genome v5.5), CDS – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) intergenic, chromosome_1 base 3186804 (genome v5.5) – WARNING position has 58% probability of being correct
2) Cre04.g217200, chromosome_4 base 202060 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
3) Cre12.g510550, chromosome_12 base 1946929 (genome v5.5), CDS – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 6 mapped insertions:
1) Cre01.g054100, chromosome_1 base 7510985 (genome v5.5), 3’UTR – WARNING position has 58% probability of being correct
2) Cre12.g547351, chromosome_12 base 8568260 (genome v5.5), CDS – WARNING position has 73% probability of being correct
3) HIK (Cre13.g571200), chromosome_13 base 1311726 (genome v5.5), intron – position has 95% probability of being correct
4) HIK (Cre13.g571200), chromosome_13 base 1311736 (genome v5.5), intron – position has 95% probability of being correct
5) Cre17.g707137, chromosome_17 base 1511726 (genome v5.5), 5’UTR – position has 95% probability of being correct
6) intergenic, chromosome_17 base 1512113 (genome v5.5) – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre16.g651150, chromosome_16 base 1253381 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre16.g651150, chromosome_16 base 1253384 (genome v5.5), CDS – position has 95% probability of being correct
3) Cre16.g651150, chromosome_16 base 1253384 (genome v5.5), CDS – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) ADC1 (Cre03.g181400), chromosome_3 base 5093567 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre06.g278111, chromosome_6 base 3545741 (genome v5.5), 5’UTR – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) CYP21,CYP743B2,CYP743B3 (Cre07.g354400), chromosome_7 base 5947544 (genome v5.5), intron – position has 95% probability of being correct
2) CYP21,CYP743B2,CYP743B3 (Cre07.g354400), chromosome_7 base 5947677 (genome v5.5), CDS – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:
1) intergenic, chromosome_3 base 7592433 (genome v5.5) – position has 95% probability of being correct
2) intergenic, chromosome_3 base 7592444 (genome v5.5) – position has 95% probability of being correct
3) Cre13.g603550, chromosome_13 base 4473435 (genome v5.5), intron – position has 95% probability of being correct
4) Cre13.g603550, chromosome_13 base 4473438 (genome v5.5), intron – position has 95% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre09.g409300, chromosome_9 base 6760639 (genome v5.5), intron – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre12.g533600, chromosome_12 base 5834618 (genome v5.5), intron – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:
1) Cre08.g362350, chromosome_8 base 1095419 (genome v5.5), 5’UTR – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:
1) Cre03.g162500, chromosome_3 base 2846368 (genome v5.5), intron – WARNING position has 73% probability of being correct
2) SHMT1 (Cre16.g664550), chromosome_16 base 2994623 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
If you use this mutant for your work, please cite: Li et al. 2019 Nature Genetics.
From the Jonikas lab, Princeton University, 2016
See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:
1) Cre03.g207351, chromosome_3 base 7265091 (genome v5.5), CDS – position has 95% probability of being correct
2) Cre03.g207351, chromosome_3 base 7265205 (genome v5.5), CDS – position has 95% probability of being correct
3) Cre04.g220576, chromosome_4 base 2312242 (genome v5.5), 3’UTR – WARNING position has 58% probability of being correct
The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.
Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A. Lefebvre, Arthur R. Grossman & Martin C. Jonikas (2019) A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics 51, 627-635.
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