# CLiP Strains

*****Prices listed are for academic and non-profit users. Commercial and industrial users are charged 1000 US$ for each strain.

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B-Series CLiP Collection
$1,000.00

$1,000.00

The B-Series CLiP Collection (called the B-series for “Best”), consists of more than 13,000 mutants selected from the CLiP collection as having a confidence level of 1 or 2 and an insertion in either the CDS and/or intron region of genes. The mutants are supplied on 9 high-density (1536 colony/plate) agar plates. Users can screen plates individually for phenotypes of interest (for example acetate auxotrophy, high light sensitivity, etc.) if they have access to a replication robot. Alternatively, users can pool the mutants from one or all of the plates, subject them to a competitive growth assay in different media or different conditions, and then identify by sequencing those mutants that were selected under desired conditions. Conditions for pooling the library and conducting competitive growth experiments are described in Li et al., 2019 (cited below). Users can replicate the plates for their own research, but only for use in their own laboratories.

Plates will be available for a cost of $1000 for the set. This resource is not provided as a low-cost way of ordering multiple mutants, but rather for phenotypic screening. Therefore, the key to the identity of individual mutants on the plates will not be provided. As strains of interest are chosen using phenotypic screens or pooled growth experiments, the user can then order the individual strains from the CLiP collection for $100 per strain.

If your laboratory has used replica plating to identify strains of interest, the identity of any individual strain will be available upon request from the CRC, at a cost of $20 per strain. Send a picture of the plate with the clone(s) of interest highlighted to learn the identity of any of the strains.

When you receive the plates, please inspect them carefully. Remove any large condensation droplets from the lid that can occur during shipping. Keep them wrapped in parafilm and put them in a plastic box upon arrival to slow desiccation of the plate. We will replace any problem plates free-of-charge within 2 weeks of receipt. Send a photo of the damaged plate to Matt Laudon immediately. For best results, you should conduct your experiments using the library no more than 2 weeks after delivery.

Because of maintenance and replication factors, please be aware there will be a small number of empty positions on the arrayed plates. Plates will be shipped approximately 2 weeks after orders are received.

List of LMJ numbers in B series plates

Li X, Patena W, Fauser F, Jinkerson RE, Saroussi S, Meyer MT, Ivanova N, Robertson JM, Yue R, Zhang R, Vilarrasa-Blasi J, Wittkopp TM, Ramundo S, Blum SR, Goh A, Laudon M, Srikumar T, Lefebvre PA, Grossman AR, Jonikas MC. A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18. PMID: 30886426; PMCID: PMC6636631.

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LMJ.RY0402.038401
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre10.g434800, chromosome_10 base 2321389 (genome v5.5), intron – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

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LMJ.RY0402.038403
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) THB3 (Cre04.g218800), chromosome_4 base 1999366 (genome v5.5), 3’UTR – position has 95% probability of being correct

2) THB3 (Cre04.g218800), chromosome_4 base 1999541 (genome v5.5), 3’UTR – position has 95% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

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LMJ.RY0402.038404
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre13.g604050, chromosome_13 base 4625016 (genome v5.5), CDS – WARNING position has 73% probability of being correct

The listed mapped insertions have a chance of being incorrect (see insertion list for details), and there may be additinal unmapped insertions. If there are two listed insertions in the same locus, they represent two sides of the same insertion. We urge you to confirm that your gene of interest is disrupted by using this PCR protocol. The insertion cassette carries paromomycin resistance, but some insertions may be of cassette fragments lacking the resistance gene.

Li X*, Zhang R*, Patena W*, Gang SS, Blum SR, Ivanova N, Yue R, Robertson JM, Lefebvre PA, Fitz-Gibbon ST, Grossman AR, Jonikas MC (2016) An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii. The Plant Cell. (* equal contribution)

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LMJ.RY0402.038407
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre17.g726850, chromosome_17 base 3682103 (genome v5.5), intron – position has 95% probability of being correct

2) Cre17.g726850, chromosome_17 base 3682106 (genome v5.5), intron – position has 95% probability of being correct

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LMJ.RY0402.038412
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:

1) Cre01.g025750, chromosome_1 base 3954555 (genome v5.5), intron – position has 95% probability of being correct

2) Cre01.g025750, chromosome_1 base 3954577 (genome v5.5), intron – position has 95% probability of being correct

3) Cre01.g025750, chromosome_1 base 3954577 (genome v5.5), intron – position has 95% probability of being correct

4) Cre01.g025750, chromosome_1 base 3954577 (genome v5.5), intron – position has 95% probability of being correct

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LMJ.RY0402.038421
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre10.g455950, chromosome_10 base 5081300 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

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LMJ.RY0402.038422
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:

1) Cre16.g695200, chromosome_16 base 148020 (genome v5.5), intron – position has 95% probability of being correct

2) Cre16.g695200, chromosome_16 base 148025 (genome v5.5), intron – position has 95% probability of being correct

3) Cre16.g690509, chromosome_16 base 3946253 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

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LMJ.RY0402.038423
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) FAP233 (Cre09.g393450), chromosome_9 base 2142911 (genome v5.5), CDS – position has 95% probability of being correct

2) FAP233 (Cre09.g393450), chromosome_9 base 2142912 (genome v5.5), CDS – position has 95% probability of being correct

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LMJ.RY0402.038424
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre03.g206900, chromosome_3 base 7306113 (genome v5.5), intron – position has 95% probability of being correct

2) Cre03.g206900, chromosome_3 base 7306168 (genome v5.5), intron – position has 95% probability of being correct

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LMJ.RY0402.038434
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre08.g384628, chromosome_8 base 4852381 (genome v5.5), 5’UTR – position has 95% probability of being correct

2) Cre08.g384628, chromosome_8 base 4852389 (genome v5.5), 5’UTR – position has 95% probability of being correct

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LMJ.RY0402.038444
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) LSM1 (Cre12.g538750), chromosome_12 base 6516940 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

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LMJ.RY0402.038445
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre10.g424000, chromosome_10 base 881539 (genome v5.5), intron – WARNING position has 73% probability of being correct

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LMJ.RY0402.038447
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:

1) Cre03.g149700, chromosome_3 base 1220231 (genome v5.5), 3’UTR – position has 95% probability of being correct

2) Cre03.g149700, chromosome_3 base 1220238 (genome v5.5), 3’UTR – position has 95% probability of being correct

3) AKC1 (Cre09.g407801), chromosome_9 base 6541203 (genome v5.5), 3’UTR – position has 95% probability of being correct

4) AKC1 (Cre09.g407801), chromosome_9 base 6541211 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038448
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre16.g675400, chromosome_16 base 6161874 (genome v5.5), 3’UTR – position has 95% probability of being correct

2) Cre16.g675400, chromosome_16 base 6161876 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038449
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) PGM19 (Cre10.g440250), chromosome_10 base 2920914 (genome v5.5), 3’UTR – position has 95% probability of being correct

2) PGM19 (Cre10.g440250), chromosome_10 base 2920915 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038451
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre01.g036200, chromosome_1 base 5201767 (genome v5.5), intron – WARNING position has 73% probability of being correct

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LMJ.RY0402.038453
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre16.g672602, chromosome_16 base 6542101 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

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LMJ.RY0402.038456
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre12.g489500, chromosome_12 base 1213830 (genome v5.5), CDS – WARNING position has 73% probability of being correct

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LMJ.RY0402.038457
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre09.g399150, chromosome_9 base 1287117 (genome v5.5), 3’UTR – position has 95% probability of being correct

2) Cre09.g399150, chromosome_9 base 1287133 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038464
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 4 mapped insertions:

1) SPA1 (Cre03.g155051), chromosome_3 base 1902457 (genome v5.5), CDS – WARNING position has 73% probability of being correct

2) Cre03.g176833, chromosome_3 base 4558364 (genome v5.5), CDS – WARNING position has 58% probability of being correct

3) PGM12 (Cre06.g293150), chromosome_6 base 6509099 (genome v5.5), 3’UTR – position has 95% probability of being correct

4) PGM12 (Cre06.g293150), chromosome_6 base 6509112 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038465
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre16.g659450, chromosome_16 base 2320697 (genome v5.5), intron – position has 95% probability of being correct

2) Cre16.g659450, chromosome_16 base 2321455 (genome v5.5), intron – position has 95% probability of being correct

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LMJ.RY0402.038466
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:

1) Cre04.g219000, chromosome_4 base 2037015 (genome v5.5), CDS – WARNING position has 73% probability of being correct

2) PKS1 (Cre10.g449750), chromosome_10 base 4111897 (genome v5.5), CDS – position has 95% probability of being correct

3) PKS1 (Cre10.g449750), chromosome_10 base 4111907 (genome v5.5), CDS – position has 95% probability of being correct

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LMJ.RY0402.038469
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre08.g383400, chromosome_8 base 4620299 (genome v5.5), CDS – WARNING position has 73% probability of being correct

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LMJ.RY0402.038472
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 3 mapped insertions:

1) CYG22 (Cre02.g100500), chromosome_2 base 4517265 (genome v5.5), 3’UTR – WARNING position has 73% probability of being correct

2) Cre16.g661626, chromosome_16 base 2596108 (genome v5.5), 3’UTR – position has 95% probability of being correct

3) Cre16.g661626, chromosome_16 base 2596113 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038473
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre14.g618300, chromosome_14 base 1494083 (genome v5.5), CDS – position has 95% probability of being correct

2) Cre14.g618300, chromosome_14 base 1494724 (genome v5.5), 3’UTR – position has 95% probability of being correct

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LMJ.RY0402.038474
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) PKS3 (Cre17.g722150), chromosome_17 base 3204227 (genome v5.5), intron – position has 95% probability of being correct

2) PKS3 (Cre17.g722150), chromosome_17 base 3204229 (genome v5.5), intron – position has 95% probability of being correct

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LMJ.RY0402.038476
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) DCL3 (Cre07.g345900), chromosome_7 base 4824427 (genome v5.5), intron – position has 95% probability of being correct

2) DCL3 (Cre07.g345900), chromosome_7 base 4824484 (genome v5.5), intron – position has 95% probability of being correct

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LMJ.RY0402.038480
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 1 mapped insertions:

1) Cre16.g679876, chromosome_16 base 3554816 (genome v5.5), intron – WARNING position has 58% probability of being correct

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LMJ.RY0402.038483
$100.00

$100.00

From the Jonikas lab, Princeton University, 2016

See the mutant page on the CLiP website for detailed insertion information. Summary: this mutant strain has 2 mapped insertions:

1) Cre16.g651400, chromosome_16 base 1282410 (genome v5.5), intron – position has 95% probability of being correct

2) Cre16.g651400, chromosome_16 base 1282417 (genome v5.5), intron – position has 95% probability of being correct

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