Strains
CC-6211 E1_hybrid
$30.00
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed).
Dataset info: https://doi.org/10.5281/zenodo.14020362
CC-6212 E2_hybrid
$30.00
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed).
Dataset info: https://doi.org/10.5281/zenodo.14020362
CC-6213 F3_hybrid
$30.00
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed).
Dataset info: https://doi.org/10.5281/zenodo.14020362
CC-6214 F5_hybrid
$30.00
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed).
Dataset info: https://doi.org/10.5281/zenodo.14020362
CC-6221 fmg1b
$30.00
$30.00
From Adrian Nievergelt, Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Potsdam, Germany, July 2025
Background: CC-124 32M
Origin: CRISPR-mediated insertional knock-out of FMG1B.
Description: This strain lacks the B-isoform of FMG1 and has no clear phenotype. Nourseothricin resistant.
Locus: FMG1B (Cre09.g392867_4532).
Hoepfner LM, Nievergelt AP, Matrino F, Scholz M, Foster HE, Rodenfels J, von Appen A, Hippler M, Pigino G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci (Weinh). 2025 Apr;12(16):e2413355. doi: 10.1002/advs.202413355. Epub 2025 Mar 5. PMID: 40041987; PMCID: PMC12021028.
CC-6222 fmg1a fmg1b
$30.00
$30.00
From Adrian Nievergelt, Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Potsdam, Germany, July 2025
Background: CC-6221
Origin: CRISPR-mediated insertional knock-out of FMG1A.
Description: This strain lacks both A and B isoforms of FMG1. The strain has altered ciliary adhesion. Nourseothricin and Spectinomycin resistant.
Locus: FMG1B (Cre09.g392867_4532, Chromosome 9), FMG1A (Cre18.g750047_4532, Chromosome 9)
Hoepfner LM, Nievergelt AP, Matrino F, Scholz M, Foster HE, Rodenfels J, von Appen A, Hippler M, Pigino G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci (Weinh). 2025 Apr;12(16):e2413355. doi: 10.1002/advs.202413355. Epub 2025 Mar 5. PMID: 40041987; PMCID: PMC12021028.
From Adrian Nievergelt, Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Potsdam, Germany, July 2025
Background: CC-124/CC-125
Origin: Cross of CC-6221 and CC-5900 to allow for observation of intraflagellar transport and gliding activity in the absence of the ciliary coat proteins FMG1A and FMG1B.
Description: This strain lacks both A and B isoforms of FMG1. The strain has altered ciliary adhesion. Intraflagellar transport is labelled for green fluorescence. Nourseothricin, Spectinomycin, and Paromomycin resistant.
Locus: FMG1B (Cre09.g392867_4532, Chromosome 9), FMG1A (Cre18.g750047_4532, Chromosome 9), IFT46 (Cre05.g241637_4532, Chromosome 5)
Hoepfner LM, Nievergelt AP, Matrino F, Scholz M, Foster HE, Rodenfels J, von Appen A, Hippler M, Pigino G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci (Weinh). 2025 Apr;12(16):e2413355. doi: 10.1002/advs.202413355. Epub 2025 Mar 5. PMID: 40041987; PMCID: PMC12021028.
From Adrian Nievergelt, Max-Planck-Institute of Molecular Plant Physiology (MPI-MP), Potsdam, Germany, July 2025
Background: CC-6012
Origin: CRISPR-mediated endogenous c-terminal mScarlet-I fusion knock-in.
Description: This strain has bright fluorescent cilia and allows for the differentiation of the expression of the ciliary coat protein isoforms FMG1A and FMG1B. Paromomycin and Nourseothricin resistant.
Hoepfner LM, Nievergelt AP, Matrino F, Scholz M, Foster HE, Rodenfels J, von Appen A, Hippler M, Pigino G. Unwrapping the Ciliary Coat: High-Resolution Structure and Function of the Ciliary Glycocalyx. Adv Sci (Weinh). 2025 Apr;12(16):e2413355. doi: 10.1002/advs.202413355. Epub 2025 Mar 5. PMID: 40041987; PMCID: PMC12021028.
CC-6225 cc1690_pJPW4_PHL7
$30.00
$30.00
From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024
Chlamydomonas reinhardtii CC-1690, transformed with the vector pJPW4_PHL7. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the fluorescent protein PHL7, which is directed to the cell wall via the SP7 signal peptide and linkage to a GP1, hydroxyproline-rich protein hypothesized to be attached to the cell wall by non-covalent interactions. A pH-sensitive intein is placed between mCherry and the GP1 protein and appears to release PHL7 from the cell wall at lower pH <6.
The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.
CC-6245 LEE-1; HygroR mt-
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Hygromycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6246 LEE-3; ParoR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Paromomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6247 LEE-3; SpecR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6248 LEE-3; BlastiR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Basticidin S-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Hygromycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6250 Harris #6; ParoR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Paromomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6251 Harris #6; SpecR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Basticidin S-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Hygromycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6254 NIES-2464; ParoR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Paromomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
CC-6255 NIES-2464; SpecR mt+
$30.00
$30.00
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025
The strain was generated by electroporation-based transformation with a plasmid conferring Blasticidin S-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).
Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.
Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.
From Sven M Lange, Harvard Medical School, April 2025
For visualization of IFT with red-fluorescent mScarlet-IFT54 and IFT-ubiquitin adaptor CFAP36 with green-fluorescent CFAP36-mStayGold. Resistant to hygromycin and blasticidin.
https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1
From Sven M Lange, Harvard Medical School, April 2025
For visualization of IFT with red-fluorescent mScarlet-IFT54 and CFAP36 IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of CFAP36 disrupts interaction of CFAP36 and IFT. Resistant to hygromycin and blasticidin.
https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1
From Sven M Lange, Harvard Medical School, April 2025
For visualization of IFT with red-fluorescent mScarlet-IFT54 and CFAP36 IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of CFAP36 disrupts interaction of CFAP36 and IFT. Resistant to hygromycin and blasticidin.
https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1
From Sven M Lange, Harvard Medical School, April 2025
For visualization of IFT with red-fluorescent mScarlet-IFT54 and CFAP36 IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of CFAP36 disrupts interaction of CFAP36 and IFT. Resistant to hygromycin and blasticidin.
https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1
From Sven M Lange, Harvard Medical School, April 2025
For visualization of IFT with red-fluorescent mScarlet-IFT54 and IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of IFT70 disrupts interaction of IFT and CFAP36. Resistant to hygromycin, blasticidin and spectinomycin.
https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1
CC-6263 pgrl1-86 mt+
$30.00
$30.00
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Proton gradient regulation like protein 1 mutant; other name: 204D1. Sensitive to short and long scale light fluctuations; high light. Hygromycin resistant.
Dao O, Burlacot A, Buchert F, Bertrand M, Auroy P, Stoffel C, Madireddi SK, Irby J, Hippler M, Peltier G, Li-Beisson Y. Cyclic and pseudo-cyclic electron pathways play antagonistic roles during nitrogen deficiency in Chlamydomonas reinhardtii. Plant Physiol. 2024 Dec 23;197(1):kiae617. doi: 10.1093/plphys/kiae617. PMID: 39560077.
CC-6264 flvA-e12 mt+
$30.00
$30.00
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Thylakoid flavodiiron protein mutant. Sensitive to short light fluctuations. Hygromycin resistant.
CC-6265 pgr5-107 mt+
$30.00
$30.00
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Proton-gradient related protein mutant. Sensitive to short light fluctuations. Hygromycin resistant.
CC-6266 pgr1-162 mt+
$30.00
$30.00
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Proton-gradient related protein mutant. Sensitive to short light fluctuations. Hygromycin resistant.