Strains

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-620 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Progeny of Chlamydomonas reinhardtii CC-1690 pJPW2_mCherry and fused to Chlamydomonas pacifica CC-5697 pJPSHx1 PHL7, from João Vitor Dutra Molino at the Stephen Mayfield Laboratory, University of California, San Diego (September 2024). This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and resistant to hygromycin due to the incorporation of the aph7” gene. It expresses PHL7 which is directed to the secretory pathway via the SP7 signal peptide and expresses the fluorescent protein mCherry (Cell wall localization was not confirmed). 

Dataset info: https://doi.org/10.5281/zenodo.14020362

From Abhishek Gupta and Joao Vitor Dutra Molino, Stephen Mayfield lab, University of San Diego, August 2024

Chlamydomonas reinhardtii CC-1690, transformed with the vector pJPW4_PHL7. This strain is resistant to zeocin due to the incorporation of the bleomycin resistance gene and expresses the fluorescent protein PHL7, which is directed to the cell wall via the SP7 signal peptide and linkage to a GP1, hydroxyproline-rich protein hypothesized to be attached to the cell wall by non-covalent interactions. A pH-sensitive intein is placed between mCherry and the GP1 protein and appears to release PHL7 from the cell wall at lower pH <6.

The strain is cultivated on either TAP (Tris-Acetate-Phosphate) or HSM (High Salt Minimal) media and can efficiently assimilate acetate as a carbon source. It utilizes various nitrogen sources, including nitrate, ammonia, and urea, making it versatile for different growth conditions.

 

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Hygromycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Paromomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Basticidin S-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Hygromycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Paromomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Basticidin S-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Hygromycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Paromomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Spectinomycin-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Antoine Van de Vloet, VIB-Ugent Center for Plant Systems Biology, March 2025

The strain was generated by electroporation-based transformation with a plasmid conferring Blasticidin S-resistance. The level-1 plasmid used is described and characterized in De Carpentier et al. (2020). Constructs used to engineer the strain were generated using the Chlamydomonas modular cloning (MoClo) toolkit as described in Crozet et al. (2018).

Van de Vloet A, Prost-Boxoen L, Bafort Q, Paing YT, Casteleyn G, Jomat L, Lemaire SD, De Clerck O, Van de Peer Y. Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii. New Phytol. 2025 Mar 21. doi: 10.1111/nph.70095. Epub ahead of print. PMID: 40116553.

Crozet P, Navarro FJ, Willmund F, Mehrshahi P, Bakowski K, Lauersen KJ, Pérez-Pérez ME, Auroy P, Gorchs Rovira A, Sauret-Gueto S, Niemeyer J, Spaniol B, Theis J, Trösch R, Westrich LD, Vavitsas K, Baier T, Hübner W, de Carpentier F, Cassarini M, Danon A, Henri J, Marchand CH, de Mia M, Sarkissian K, Baulcombe DC, Peltier G, Crespo JL, Kruse O, Jensen PE, Schroda M, Smith AG, Lemaire SD. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth Biol. 2018 Sep 21;7(9):2074-2086. doi: 10.1021/acssynbio.8b00251. Epub 2018 Sep 5. PMID: 30165733.

From Sven M Lange, Harvard Medical School, April 2025

For visualization of IFT with red-fluorescent mScarlet-IFT54 and IFT-ubiquitin adaptor CFAP36 with green-fluorescent CFAP36-mStayGold. Resistant to hygromycin and blasticidin.

https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1

  • Locus:
  • IFT54, CFAP36
  • Chromosome:
  • 11, 9

From Sven M Lange, Harvard Medical School, April 2025

For visualization of IFT with red-fluorescent mScarlet-IFT54 and CFAP36 IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of CFAP36 disrupts interaction of CFAP36 and IFT. Resistant to hygromycin and blasticidin.

https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1

  • Locus:
  • IFT54, CFAP36
  • Chromosome:
  • 11, 9

From Sven M Lange, Harvard Medical School, April 2025

For visualization of IFT with red-fluorescent mScarlet-IFT54 and CFAP36 IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of CFAP36 disrupts interaction of CFAP36 and IFT. Resistant to hygromycin and blasticidin.

https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1

  • Locus:
  • IFT54, CFAP36
  • Chromosome:
  • 11, 9

From Sven M Lange, Harvard Medical School, April 2025

For visualization of IFT with red-fluorescent mScarlet-IFT54 and CFAP36 IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of CFAP36 disrupts interaction of CFAP36 and IFT. Resistant to hygromycin and blasticidin.

https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1

  • Locus:
  • IFT54, CFAP36
  • Chromosome:
  • 11, 9

From Sven M Lange, Harvard Medical School, April 2025

For visualization of IFT with red-fluorescent mScarlet-IFT54 and IFT-ubiquitin adaptor with green-fluorescent CFAP36-mStayGold. Targeted mutation of IFT70 disrupts interaction of IFT and CFAP36. Resistant to hygromycin, blasticidin and spectinomycin.

https://www.biorxiv.org/content/10.1101/2025.04.24.650332v1

  • Locus:
  • IFT54, CFAP36, IFT70
  • Chromosome:
  • 11, 9, 7