Strains
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Thylakoid flavodiiron protein mutant. Sensitive to short light fluctuations. Hygromycin resistant.
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Thylakoid flavodiiron protein mutant. Sensitive to short light fluctuations. Hygromycin resistant.
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Proton gradient regulation protein mutant; chloroplast protein kinase required for state transitions protein mutant. Sensitive to short and long scale light fluctuations; high light. Hygromycin resistant.
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Proton gradient regulation like protein 1 mutant; chloroplast protein kinase required for state transitions protein mutant. Sensitive to short and long scale light fluctuations; high light. Hygromycin resistant.
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
Proton-gradient related protein mutant. Sensitive to short and long scale light fluctuations; high light. Hygromycin resistant.
From Andrea Rico Montana, Adrien Burlacot lab, Carnegie Institution for Science, May 2025
State transition kinase mutant. Hygromycin resistant.
N-terminal region of PetD is essential for cytochrome b6f function and controls STT7 kinase activity via STT7-dependent feedback loop phosphorylation
Afifa Zaeem, Yuval Milrad, Simon Bütfering, Carolyne Stoffel, Adrien Burlacot, Martin Scholz, Felix Buchert, Michael Hippler
bioRxiv 2025.02.21.639470; doi: https://doi.org/10.1101/2025.02.21.639470
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: HLM6 (Cre02.g116850) – Histone Lysine Methyltransferase (putative)
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: HLM14 (Cre08.g385300) – Histone Lysine Methyltransferase (putative)
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: HLM25 (Cre16.g664000) – Histone Lysine Methyltransferase (putative)
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: SRTB (Cre12.g524650) – Sir2-Type Histone Deacetylase (putative)
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: SRTC (Cre05.g234663) – Sir2-Type Histone Deacetylase (putative)
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR
Initial KO target: MUT11 (Cre08.g367650) – WD40-repeat-containing protein, subunit of Histone Methyltransferase Complexes
This strain requires arginine supplementation for maintenance.
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: SRTA (Cre10.g462200)- Sir2-Type Histone Deacetylase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Second KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Second KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component
Second KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Second KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase (putative)
Second KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component
Second KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7
Initial KO target: VIG1 (Cre09.g393358) – Orthologue of the Vasa intronic gene, ancillary ribosomal component
Second KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Third KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Second KO target: DMC5 (Cre10.g461750) – DNMT1-type cytosine-C5 DNA methylase
Third KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.
From Alexander Einhaus, Algae Biotechnology and Bioenergy, Bielefeld University, Germany, June 2025
Background: CC-4350
Origin: CRISPR Cas9-mediated integration of selectable marker
Selectable marker: HygR, Arg7, NourR
Initial KO target: SRTA (Cre10.g462200) – Sir2-Type Histone Deacetylase
Second KO target: HLM4 (Cre02.g089200) – Histone Lysine Methyltransferase
Third KO target: MUT9 (Cre06.g281100) – Serine/Threonine Protein Kinase
Einhaus A, Krieger A, Köhne L, Rautengarten B, Jacobebbinghaus N, Saudhof M, Baier T, Kruse O. Genome editing of epigenetic transgene silencing in Chlamydomonas reinhardtii. Trends Biotechnol. 2025 May 30:S0167-7799(25)00164-7. doi: 10.1016/j.tibtech.2025.04.019. Epub ahead of print. PMID: 40450408.