The CRC is pleased to announce the availability of the Modular Cloning Kit (“MoClo Kit”) from Crozet et al. (2018)

Crozet et al. (2018) Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synthetic Biology 7:2074–2086

From the paper: “… we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.”

The entire collection of plasmids, distributed as frozen glycerol stocks in a 96-well format, will be priced at $400 for academic/non-profit users and $800 for commercial/industry.  Inpidual plasmids from the kit can also be ordered as bacterial cultures in agar plugs for standard plasmid pricing.

It will be a few weeks until we upload all of the plasmids to the website. Until then, please contact us and we can place your order manually.

Chlamy MoClo Core Kit – list of plasmids