The version 2 microarray is ready for shipping. This array consists of about 10,000 oligonucleotides, each approximately 70 nucleotides long. Each of the oligonucleotide should represent a unique gene. The set of 10,000 oligonucleotides was printed twice on the same slide (so each slide has duplicates). The oligonucleotides were prepared by Integrated DNA Technologies and their quality was checked by mass spectroscopy. The set of ‘unigenes sequences’ used for oligonucleotide generation was developed by Monica Jain and Stephan Eberhard in my laboratory. We will also provide information describing potentially empty and weak spots (there were very few of these in our initial examination). While the .gal file with a gene identifier and the sequence of the oligonucleotide used at each position on the array will be available, it may take a little more time to link whatever annotation is available to the individual spots (it will be a priority over the next month or two). We will also put a protocol onto the web site that we used for the hybridizations and will try to address any technical questions that you might have concerning the array. A manuscript describing how the unigene set was chosen and oligonucleotide design is in preparation, and we will make it available to people using the array as soon as possible.
The cost is $100 per slide. Please send orders to:
Department of Plant Biology
Carnegie Institution of Washington
260 Panama Street
Stanford, CA 94305
Telephone: 650 325-1521 X 212
If you have any questions contact me by Email or phone.