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CC-5499 ∆ChR1 ∆ChR2 [PH128]

Name: CC-5499 ∆ChR1 ∆ChR2 [PH128]
SKU: CC-5499 ∆ChR1 ∆ChR2 [PH128]
Price: 30.00 USD
Availability: InStock

$30.00

Deposited by Olga Baidukova, Peter Hegemann lab, Humboldt University-Berlin, March 2019

This is a ChR1 /ChR2 disruption strain, generated with CRISPR/Cas9.

Background strain :    CC-125
Nuclease:                               (Sp)Cas9 as ribonucleoprotein (RNP)
Marker:                                  pAPHVIII (p114), pAPHVII (p360)
Target gene:                          ChR1 (COP3), Cre14.g611300
ChR2 (COP4), Cre02.g085257
Target sequence : ChR1: TGTGGCTTCGTTACGCGGAGTGG (Exon5)
ChR2: GCTCGCGCCCAACGGCACTCAGG (Exon1)

Overview of all CRISPR/Cas9 strains from the Hegemann lab
http://www.chlamy.de/strains

Visit www.chlamy.de for more info or contact CRISPR@chlamy.de

This is an unpublished strain. Please contact ph@chlamy.de before using it.

Locus:
ChR1, ChR2
Chromosome:
14, 2
Mutation Information:
Predicted Mutation Effect:

CC-5499 ∆ChR1 ∆ChR2 [PH128]

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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