CC-6027 cLM307_EPYC1-TurboID-3xHA mt-


From Chun Sing Lau, Mackinder lab, University of York, July 2023

This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of EPYC1 (Cre10.g436550) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.

Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.

Out of stock