Venus & FLAG-tagged Plasmids for Chlamydomonas Spatial Interactome (CSI) Collection, Wang/Jonikas 2022
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
From Martin Jonikas, Princeton University, March 2023
This plasmid was generated by cloning genes into pLM005 which has ampicillin and paromomycin resistance cassettes. The Venus-3xFLAG tagged gene is driven by PSAD promoter and PSAD terminator.
Host strain: NEB 5-alpha Competent E. coli
Selection in E. coli: ampicillin
A Chloroplast Protein Atlas Reveals Novel Structures and Spatial Organization of Biosynthetic Pathways
Lianyong Wang, Weronika Patena, Kelly A. Van Baalen, Yihua Xie, Emily R. Singer, Sophia Gavrilenko, Michelle Warren-Williams, Linqu Han, Henry R. Harrigan, Vivian Chen, Vinh T.N.P. Ton, Saw Kyin, Henry H. Shwe, Matthew H. Cahn, Alexandra T. Wilson, Jianping Hu, Danny J. Schnell, Claire D. McWhite, Martin Jonikas
bioRxiv 2022.05.31.493820; doi: https://doi.org/10.1101/2022.05.31.493820
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