Strains

Hurley Shepherd in Boynton-Gillham laboratory, Duke University

Phenotype: requires acetate; antibiotic resistant (spectinomycin)

From a cross of the original ac-u-b-1-10 mutant (CC-366, no longer in the collection) to an spr-u-1-27-3 strain derived from the precursor of CC-104.

The ac-u-b-1-10 mutant is deficient in ATP synthase activity, but the mutational lesion has not been identified. Chloroplast DNA appears normal when digested with BamHI or EcoRI.

The spr-u-1-27-3 mutation confers spectinomycin resistance. See CC-104 for additional information.

Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of
Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357

Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of
Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151

• Locus:
• rrnS
• Chromosome:
• chloroplast

Hurley Shepherd in Boynton-Gillham laboratory, Duke University

Phenotype: requires acetate; antibiotic resistant (spectinomycin)

The ac-u-i-2-25 mutant is deficient in chloroplast ATP synthase activity. The original mutant strain studied by Shepherd also lacked chlorophyll protein complex CP I and was originally thought to have a primary defect in this protein. Subsequent analysis by Bennoun (see Woessner et al., 1984) showed that this mutant has a leaky ATP synthase-deficient phenotype in dim light.

This information was published in a footnote to a table in Woessner et al. (1984); however this mutant was not included in the complementation/recombination matrix in that paper.

This mutation is closely linked genetically to Bennoun’s CP I deficient mutant C1.

See CC-104 for additional information on the spr-u-1-27-3 mutant.

Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357

Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190

• Locus:
• rrnS
• Chromosome:
• chloroplast

Hurley Shepherd in Boynton-Gillham laboratory, Duke University

Phenotype: requires acetate

This is a point mutation in the atpB gene, changing Lys 154 to Asn.

Shepherd HS, Boynton JE, Gillham NW (1979) Mutations in nine chloroplast loci of Chlamydomonas affecting different photosynthetic functions. Proc Natl Acad Sci USA 76:1353-1357

Myers AM, Grant DM, Rabert DK, Harris EH, Boynton JE, Gillham NW (1982) Mutants of Chlamydomonas reinhardtii with physical alterations in their chloroplast DNA. Plasmid 7:133-151

Woessner JP, Masson A, Harris EH, Bennoun P, Gillham NW, Boynton JE (1984) Molecular and genetic analysis of the chloroplast ATPase of chlamydomonas. Plant Mol Biol 3:177-190

Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240:1534-1538

Robertson D, Woessner JP, Gillham NW, Boynton JE (1989) Molecular characterization of two point mutants in the chloroplast atpB gene of the green alga Chlamydomonas reinhardtii defective in assembly of the ATP synthase complex. J Biol Chem 264:2331-2337

• Locus:
• atpB
• Chromosome:
• chloroplast

From Maureen Hanson, Bogorad lab, Harvard University, February 1978

This is a mutation that suppresses the erythromycin resistance of ery1 mutants. It was obtained in Bogorad’s laboratory by ethyl methanesulfonate mutagenesis of a stock of ery1b. Davidson mapped this to a locus now designated ES1, linked to ERY1 on chromosome 11. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 157:39-46

• Locus:
• ES1
• Chromosome:
• 11

From Maureen Hanson, Bogorad lab, Harvard University, February 1978

This is a mutation that suppresses the erythromycin resistance of ery1 mutants. It was obtained in Bogorad’s laboratory by ethyl methanesulfonate mutagenesis of a stock of ery1b. We do not have this mutant in combination with ery1b, however. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 157:39-46

From Maureen Hanson, Bogorad lab, Harvard University, February 1978

Phenotype: antibiotic resistant, suppressed (erythromycin)

This is the original es-101 strain, containing both the ery1b mutation and the suppressor. For the separate mutants, please see CC-445 and CC-942. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet. 157:39-46

• Locus:
• ES1
• Chromosome:
• 11

From Laurens Mets, Case Western Reserve University February 1978

This is a suppressor of erythromycin resistance, induced in a stock of ery1b in Bogorad’s laboratory. It appears to affect other ery mutations in addition to ery1; for example, the double mutant es-5 er-u-37 is more sensitive to erythromycin than either mutant alone. See CC-458 for the same mutation in mt-, and CC-461 for the double mutant with ery1b. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 157:39-46

Davidson JN, Hanson MR, Bogorad L (1978) Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics 89:281-297

From Laurens Mets, Case Western Reserve University February 1978

Please see CC-457 for more information on the es-5 mutation.

From Laurens Mets, Case Western Reserve University February 1978

This is a mutation that suppresses the erythromycin resistance of ery1 mutants. It was obtained in Bogorad’s laboratory by ethyl methanesulfonate mutagenesis of ery1b. See CC-946 for the same mutation in mt+, and CC-460 for the double mutant with ery1b. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 157:39-46

From Laurens Mets, Case Western Reserve University February 1978

Phenotype: antibiotic resistant, suppressed (erythromycin)

This is the original es-115 strain, containing both the ery1b mutation and the suppressor. See CC-459 and CC-942 for more information on these two mutations. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 157:39-46

From Laurens Mets, Case Western Reserve University February 1978

Phenotype: antibiotic resistant, suppressed (erythromycin)

This is the original es-5 strain, containing both the ery1b mutation and the suppressor. See CC-457 and CC-942 for more information on these two mutations. For more information on the ERY1 locus, please see CC-504.

Davidson JN and Bogorad L (1977) Suppression of erythromycin resistance in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 157:39-46

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

This mutant was induced in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. It was subsequently shown to be a mutation at the SAG1 locus. This locus is not linked to mating type, but its mutants are sex-limited, expressed only in mt+ cells.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

• Locus:
• IMP2 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

The imp2 mutation was induced in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. This isolate was recovered by forced mating of the original mutant (see CC-463).

• Locus:
• IMP2 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Cells with the imp3 mutation agglutinate strongly with partners of opposite mating type, but the initial adhesion is lost, cell walls are not shed, and gamete fusion is rare. Saito et al. have shown this mutant to be defective in production of cAMP in response to flagellar adhesion.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Saito T, Small L, Goodenough UW (1993) Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat. J Cell Biol 122:137-147

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Cells with the imp3 mutation agglutinate strongly with partners of opposite mating type, but the initial adhesion is lost, cell walls are not shed, and gamete fusion is rare. Saito et al. have shown this mutant to be defective in production of cAMP in response to flagellar adhesion.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Saito T, Small L, Goodenough UW (1993) Activation of adenylyl cyclase in Chlamydomonas reinhardtii by adhesion and by heat. J Cell Biol 122:137-147

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Cells of this mutant agglutinate, but fusion is rare.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Cells of this mutant agglutinate, but fusion is rare.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

This mutant was induced by Goodenough et al. in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. It was subsequently shown to be a mutation at the SAG 1 locus. This locus is not linked to mating type, but its mutants are sex-limited, expressed only in mt+ cells.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

• Locus:
• IMP5 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

The imp5 mutation was induced by Goodenough et al. in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. This isolate was recovered by forced mating of the original mutant (see CC-469).

• Locus:
• IMP5 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

This mutant was induced by Goodenough et al. in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. It was subsequently shown to be a mutation at the SAG 1 locus. This locus is not linked to mating type, but its mutants are sex-limited, expressed only in mt+ cells.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

• Locus:
• IMP6 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

This mutant was induced by Goodenough et al. in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. It was subsequently shown to be a mutation at the SAG 1 locus. This locus is not linked to mating type, but its mutants are sex-limited, expressed only in mt+ cells.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

• Locus:
• IMP7 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

The imp7 mutation was induced by Goodenough et al. in a mt+ strain. It does not mate, and fails to agglutinate with cells of either mating type. This isolate was recovered by forced mating of the original mutant (see CC-472).

• Locus:
• IMP7 [SAG1]
• Chromosome:
• 8

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Mutants at the SAG2 locus are sex-limited, expressed only in mt+ cells although the locus is not linked to mating type. Cells can be mated by artificial agglutination with anti-flagellar antiserum.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Mutants at the SAG2 locus are sex-limited, expressed only in mt+ cells although the locus is not linked to mating type. Cells can be mated by artificial agglutination with anti-flagellar antiserum.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

From Ursula Goodenough, Harvard University, March 1978

Phenotype: does not mate

Mutants at the SAG2 locus are sex-limited, expressed only in mt+ cells although the locus is not linked to mating type. Cells can be mated by artificial agglutination with anti-flagellar antiserum.

Goodenough UW, Hwang C, Martin H (1976) Isolation and genetic analysis of mutant strains of Chlamydomonas reinhardi defective in gametic differentiation. Genetics 82:169-186

Goodenough UW, Hwang C, Warren AJ (1978) Sex-limited expression of gene Loci controlling flagellar membrane agglutination in the chlamydomonas mating reaction. Genetics 89:235-243

Adair WS, Hwang C, Goodenough UW (1983) Identification and visualization of the sexual agglutinin from the mating-type plus flagellar membrane of Chlamydomonas. Cell 33:183-193

From Ursula Goodenough, Harvard University, March 1978

Phenotype: lacks flagella, does not mate

The bld or bald mutants were recovered as nonmating mutants in Goodenough’s laboratory, and were subsequently shown to lack flagella. This is the original bld1 mutant.

The BLD1 locus encodes intraflagellar transport protein IFT52, a homolog of C. elegans osm-6.

Pasquale SM, Goodenough UW (1987) Cyclic AMP functions as a primary sexual signal in gametes of Chlamydomonas reinhardtii. J Cell Biol 105:2279-2292

Brazelton WJ, Amundsen CD, Silflow CD, Lefebvre PA (2001) The bld1 mutation identifies the Chlamydomonas osm-6 homolog as a gene required for flagellar assembly. Curr Biol 11:1591-1594

Deane JA, Cole DG, Seeley ES, Diener DR, Rosenbaum JL (2001) Localization of intraflagellar transport protein IFT52 identifies basal body transitional fibers as the docking site for IFT particles. Curr Biol 11:1586-1590

• Locus:
• BLD1 [IFT52]
• Chromosome:
• 4

From Ursula Goodenough, Harvard University, March 1978

Phenotype: lacks flagella, does not mate

The bld or bald mutants were recovered as nonmating mutants in Goodenough’s laboratory, and were subsequently shown to lack flagella.

The bld2 mutant was characterized as defective in basal body formation, but having normal levels of centrin and a stable nucleus-basal body connector. Dutcher et al. showed that the defect is in the gene encoding epsilon tubulin. The bld2-1 allele has a premature stop codon in this gene.

Goodenough UW, St. Clair HS (1975) BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii. J Cell Biol 66:480-491

Ehler LL, Holmes JA, Dutcher SK (1995) Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies. Genetics 141:945-960

Preble AM, Giddings TH Jr, Dutcher SK (2001) Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii. Genetics 157:163-181

Dutcher SK, Morrissette NS, Preble AM, Rackley C, Stanga J (2002) Epsilon-tubulin is an essential component of the centriole. Mol Biol Cell 13:3859-3869

• Locus:
• BLD2 [TUE]
• Chromosome:
• 3

From Ursula Goodenough, Harvard University, March 1978

Phenotype: lacks flagella, does not mate

The bld or bald mutants were recovered as nonmating mutants in Goodenough’s laboratory, and were subsequently shown to lack flagella.

Please see CC-478 for more information on the bld2 mutation.

• Locus:
• BLD2 [TUE]
• Chromosome:
• 3

From Ursula Goodenough, Harvard University, March 1978

Phenotype: lacks flagella, does not mate

The bld or bald mutants were recovered as nonmating mutants in Goodenough’s laboratory, and were subsequently shown to lack flagella. The genetic lesion in this mutant has not been identified.

From Ursula Goodenough, Harvard University, March 1978

Phenotype: lacks flagella, does not mate

The bld or bald mutants were recovered as nonmating mutants in Goodenough’s laboratory, and were subsequently shown to lack flagella. The genetic lesion in this mutant has not been identified.