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P-322 cpDNA EcoRI-XhoI (5.7 kb) DCMU4 er-u-1a

Name: P-322 cpDNA EcoRI-XhoI (5.7 kb) DCMU4 er-u-1a
SKU: P-322
Price: 30.00 USD
Availability: InStock

$30.00

Boynton-Gillham laboratory, Duke University, 1991

Chloroplast DNA EcoRI-XhoI (5.7 kb) in BSKS+; psbA exon 5 with DCMU4 mutation, 5S, and 23S 3′ end with er-u-1a mutation

host strain: JM109
amp resistant

More information

Strain: CC-2534

Insert: chloroplast DNA EcoRI-XhoI (5.7 kb)

Genes present: psbA (intron 4, exon 5); 5S rRNA, 23S rRNA (3’ end)

Vector: BSKS+

Bacterial host strain: JM109

Selectable marker: amp-r, white on X-gal

Origin: Newman, 1/1991

CC-2534 = DCMU-4 er-u-1a. DCMU-4 mutation in 5th exon of C. reinhardtii psbA gene causes ser -> ala change at aa 264 D1 protein, conferring resistance to DCMU and atrazine; er-u-1a mutation is C ->T change at bp 2622 in C. reinhardtii 23S rRNA (Lemieux numbering, equivalent to E. coli 2611), conferring resistance to erythromycin.

P-322 cpDNA EcoRI-XhoI (5.7 kb) DCMU4 er-u-1a

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Banner Photo Credit: Chlamydomonas reinhardtii zygotes were stained for immunofluorescence with anti-acetylated tubulin (green), anti-phospholipase D (red), and DAPI (blue) by Karl F. Lechtreck (University of Georgia) and George B. Witman (University of Massachusetts Medical School).

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