From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g199535-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g255300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g436550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g293850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre01.g054850-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g519300-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g685000-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g485050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g120100-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre12.g516450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre06.g251150-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g651050-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g725500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre05.g243300-Venus-3xFLAG (Gene Cre05.g243300 in an old gene model [Augustus v10.2 ID] and has no current equivalent gene model)

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre17.g724550-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre09.g394473-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre09.g415700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g452800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g162800-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre05.g248400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre05.g248450-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g080700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre13.g603700-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre09.g403900-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre03.g202400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre08.g379650-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre02.g145950-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre10.g439500-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.

From Martin Jonikas, Princeton University, April 2017

cMJ030 expressing pLM005-Cre16.g663400-Venus-3xFLAG

The Chlamydomonas Spatial Interactome (CSI) collection was generated by Luke Mackinder/Martin Jonikas. The collection contains strains expressing a single Venus YFP tagged protein (CSI_FC prefix) or two different tagged proteins: Venus YFP and mCherry (CSI_DT prefix).

Target genes, primarily implicated in the Chlamydomonas CCM, were cloned into the pLM005 (Venus) or pLM006 (mCherry) vectors and transformed into strain cMJ030 (CC-4533). Lines were then selected for positive fluorescence. Further details are available in the below publications and at https://sites.google.com/site/chlamyspatialinteractome/ .

To minimize silencing we recommend propagating the CSI_FC strains on paromomycin and the CSI_DT strains on paromomycin and hygromycin.