Plasmids

From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020

Recombineering plasmid designed for generating C-terminal CrVenusYFP-3xFLAG fusion proteins.

Host strain: DB3.1 (streptomycin resistant, ccdB resistant)
Selection in E. coli: kanamycin
Selection in C. reinhardtii: hygromycin

Sequence

https://www.biorxiv.org/content/10.1101/2020.05.06.080416v1

From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020

Recombineering plasmid designed for generating C-terminal mScarlet-i-3xFLAG fusion proteins.

Host strain: DB3.1 (streptomycin resistant, ccdB resistant)
Selection in E. coli: kanamycin
Selection in C. reinhardtii: hygromycin

Sequence

https://www.biorxiv.org/content/10.1101/2020.05.06.080416v1

From Gary Yates, Luke Mackinder lab, University of York-UK, July 2020

Recombineering plasmid designed for generating C-terminal mTurquoise2-3xHA fusion proteins.

Host strain: DB3.1 (streptomycin resistant, ccdB resistant)
Selection in E. coli: kanamycin
Selection in C. reinhardtii: zeocin

Sequence

https://www.biorxiv.org/content/10.1101/2020.05.06.080416v1