Strains

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: antibiotic resistant (erythromycin)

The ery2e mutant was isolated in the Boynton/Gillham laboratory as ery12, induced by exposure to 50 micrograms/ml nitrosoguanidine, selected on 100 microgram/ml ery-HS plates.

The ery2 mutants as a group are resistant to 100 micrograms/ml erythromycin on minimal medium, and are cold-sensitive. Please see CC-1711 for more information on the ERY2 locus.

Mets LJ, Bogorad L (1971) Mendelian and uniparental alterations in erythromycin binding by plastid ribosomes. Science 174:707-709

Mets LJ, Bogorad L ( 1972) Altered chlorplast ribosomal proteins associated with erythromycin-resistant mutants in two genetic systems of Chlamydomonas reinhardi. Proc Natl Acad Sci USA 69:3779-3783

Davidson JN, Hanson MR, Bogorad L (1978) Erythromycin resistance and the chloroplast ribosome in Chlamydomonas reinhardi. Genetics 89:281-297

Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353

  • Locus:
  • ERY2 [RPL22]
  • Chromosome:
  • 13

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

Cells of the pf10 mutant swim with a jiggling motion and travel in small irregular circles. This abnormal swimming results from a change to a nearly symmetric flagellar beat pattern, which is conditionally dependent on light intensity. The mutant is very easy to score. A number of suppressor mutations have been characterized by Dutcher and colleagues.

Ramanis Z, Luck DJ (1986) Loci affecting flagellar assembly and function map to an unusual linkage group in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 83:423-426

Dutcher SK, Gibbons W, Inwood WB (1988) A genetic analysis of suppressors of the PF10 mutation in Chlamydomonas reinhardtii. Genetics 120:965-976

  • Locus:
  • PF10
  • Chromosome:
  • 17

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using acridine orange as mutagen. It has abnormal motility.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using acridine orange as mutagen. It does not swim, and the flagella are at abnormal angles.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using acridine orange as mutagen. Its swimming is abnormal.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It was reported to have impaired motility but was not characterized further.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. Its flagella are at abnormal angles.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It was reported to have impaired motility but was not characterized further.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has swollen flagella.

McVittie A ( 1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen, and was originally designated NG13, the name under which we received it from CCAP. It has long flagella and was subsequently renamed lf2. CC-803 is the same allele and is the strain that we normally distribute for lf2. Please see the notes on that strain for additional information and reference citations on the LF2 locus.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

McVittie A (1972) Genetic studies on flagellum mutants of Chlamydomonas reinhardtii. Genet Res 19:157-164

  • Locus:
  • LF2
  • Chromosome:
  • 12

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has abnormal motility.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. Its motility was reported to be impaired, but it was not characterized further. In our tests CC-914 swam slowly.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It lacks flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This is a spontaneous mutant isolated by McVittie in the 137c background. It has swollen flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. Its flagella are at abnormal angles.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It was described as having impaired motility but was not characterized further.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It was reported to have impaired motility but was not characterized further. In our tests CC-922 swam, but slowly and somewhat abnormally.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. Its motility was reported to be impaired, but it was not characterized further. In our tests CC-923 swam slowly.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

McVittie A ( 1972) Genetic studies on flagellum mutants of Chlamydomonas reinhardtii. Genet Res 19:157-164

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It lacks flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has abnormal motility.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

McVittie A (1972) Genetic studies on flagellum mutants of Chlamydomonas reinhardtii. Genet Res 19:157-164

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. Its motility was reported to be impaired, but it was not characterized further. In our tests, cultures of CC-926 were mostly paralyzed, with a few cells that swam slowly.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

McVittie A (1972) Genetic studies on flagellum mutants of Chlamydomonas reinhardtii. Genet Res 19:157-164

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It was described as having abnormal motility but was not characterized further.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This is mutant defective in the flagellar cental pair is easily scored; the cells jiggle but do not swim. Please see CC-1029 for more information on the PF6 locus.

  • Locus:
  • PF6
  • Chromosome:
  • 10

Culture Centre of Algae and Protozoa (CCAP), January 1980

CCAP obtained this strain from Warr, who recovered the mutation in a stock of pf19B. The original description states that it partially suppresses paralysis of pf15 and pf19, with slight suppression of pf18.

Warr JR, McVittie A, Randall JT, Hopkins JM (1966) Genetic control of flagellar structure in Chlamydomonas reinhardtii. Genet Res 7:335-351

From Jacques Garnier, CNRS, Gif-sur-Yvette, February 1980

Phenotype: requires acetate

This is a nuclear non-photosynthetic mutant, deficient in both cytochrome and photosystem I activity.

Garnier J, Guyon D, Picaud A (1979) Characterization of new strains of non-photosynthetic mutants of Chlamydomonas reinhardtii. I: fluorescence, photochemical activities, chlorophyllprotein complexes. Plant Cell Physiol 20:1013–1027

Maroc J and Garnier J (1973) Studies of cytochrome b-563 and P700 in three non-photosynthetic mutants of Chlamydomonas reinhardti. Biochim Biophys Acta 292:477-490

Maroc J and Garnier J (1975) Cytochrome b-559 photooxidation in the presence of carbonyl cyanide p-trifluorometh-oxyphenylhydrazone and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone or p-benzoquinone in three non-photosynthetic mutants of Chlamydomonas reinhardti. Biochim Biophys Acta 387:52-68

From Jacques Garnier, CNRS, Gif-sur-Yvette, February 1980

Phenotype: requires acetate

This is a nuclear non-photosynthetic mutant, deficient in both cytochrome and photosystem I activity. Please see CC-937 for additional information.

From Maureen Hanson, University of Virginia, February 1980

Phenotype: antibiotic resistant (erythromycin)

The ery1a mutation was isolated in Bogorad’s laboratory in the 137c background, induced by mutagenesis with ethyl methanesulfonate.

The ery1 mutations were shown by Davidson et al. (1974) to alter a protein of the large subunit of the chloroplast ribosome, subsequently identified by Bowers et al. as the product of the RPL4 gene.

Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132:119-129

Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353

  • Locus:
  • ERY1 [PRPL4]
  • Chromosome:
  • 11

From Maureen Hanson, University of Virginia, February 1980

Phenotype: antibiotic resistant (erythromycin)

The ery1b mutation was isolated in Bogorad’s laboratory in the 137c background, induced by mutagenesis with ethyl methanesulfonate. This strain has been used in many crosses by the Chlamydomonas Center and has been well-behaved, easy to score.

The ery1 mutations were shown by Davidson et al. (1974) to alter a protein of the large subunit of the chloroplast ribosome, subsequently identified by Bowers et al. as the product of the RPL4 gene. The ery1b allele results in an L4 protein that is about 30% smaller than the comparable wild-type protein.

Davidson JN, Hanson MR, Bogorad L (1974) An altered chloroplast ribosomal protein in ery-M1 mutants of Chlamydomonas reinhardi. Mol Gen Genet 132:119-129

Bowers AK, Keller JA, Dutcher SK (2003) Molecular markers for rapidly identifying candidate genes in Chlamydomonas reinhardtii. Ery1 and ery2 encode chloroplast ribosomal proteins. Genetics 164:1345-1353

  • Locus:
  • ERY1 [PRPL4]
  • Chromosome:
  • 11