Strains

From Jacqueline Hermann-Smith, University of Swansea, November 1979

The nitB or nit-B mutation was isolated in Sager’s 21 gr strain by Syrett and colleagues. It lacks both the diaphorase and terminal reductase activities of the nitrate reductase complex.

Nichols GL, Syrett PJ (1978) Nitrate reductase deficient mutants of Chlamydomonas reinhardii. Isolation and genetics. J Gen Microbiol 108:71–77

Nichols GL, Shehata SAM, Syrett PJ (1978) Nitrate reductase deficient mutants of Chlamydomonas reinhardii. Biochemical characteristics. J Gen Microbiol 108:79–88

  • Locus:
  • NIT2
  • Chromosome:
  • 3

Boynton-Gillham laboratory, Duke University, November 1979

Phenotype: antibiotic resistant (spectinomycin)

This strain was obtained from a cross of CC-770 to wild type CC-124, and was used in subsequent mapping crosses as a marker for linkage group XVIII.

  • Locus:
  • SPR1
  • Chromosome:
  • 16

From George Witman, Princeton University, December 1979

This mutant has abnormally long flagella, and swims slowly. The flagella are slow to regenerate after deflagellation.

This is the original lf1 mutant, isolated by McVittie in the 137c wild type background.

The LF1 locus corresponds to a gene encoding a calcium-binding EF-hand protein involved in the regulation of flagellar length. The protein is found in the cell body in the Length Regulatory Complex (LRC).

Barsel SE, Wexler DE, Lefebvre PA (1988) Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics 118:637-648

Tam LW, Wilson NF, Lefebvre PA (2007) A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas. J Cell Biol 176:819-829

  • Locus:
  • LF1
  • Chromosome:
  • 2

From George Witman, Princeton University, December 1979

This mutant has abnormally long flagella, and swims slowly. The flagella are slow to regenerate after deflagellation.

This is the original lf2 mutant, isolated by McVittie by nitrosoguanidine mutagenesis in the 137c wild type background, and was originally designated NG13. Other alleles are known at this locus: see CC-2287, CC-2288, and CC-2973.

The LF2 locus corresponds to the CDKI1 gene encoding a novel cyclin-dependent kinase required for control of flagellar length. The protein is found predominantly, if not exclusively, in the cell body in punctate structures call the Length Regulatory Complex (LRC).

Barsel SE, Wexler DE, Lefebvre PA (1988) Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii. Genetics 118:637-648

Tam LW, Wilson NF, Lefebvre PA (2007) A CDK-related kinase regulates the length and assembly of flagella in Chlamydomonas. J Cell Biol 176:819-829

  • Locus:
  • LF2
  • Chromosome:
  • 12

From George Witman, Princeton University, December 1979

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Jarvik JW, Chojnacki B (1985) Flagellar morphology in stumpy-flagella mutants of Chlamydomonas reinhardtii. J Protozool 32:649-656

From George Witman, Princeton University, December 1979

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71, 525-540

Jarvik JW, Chojnacki B (1985) Flagellar morphology in stumpy-flagella mutants of Chlamydomonas reinhardtii. J Protozool 32:649-656

From George Witman, Princeton University, December 1979

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It has short flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

From George Witman, Princeton University, December 1979

Phenotype: impaired motility

The pf15 mutant was isolated by Lewin. pf15a was a clone of the original mutant that grew better and showed cleaner 2:2 segregation. pf15b was an allelic mutation isolated by Warr et al. All the pf15 isolates currently in our collection are probably the pf15a clone.

For more information on the PF15 locus, please see CC-1033.

  • Locus:
  • PF15 [KAT2]
  • Chromosome:
  • 3

From George Witman, Princeton University, December 1979

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

From George Witman, Princeton University, December 1979

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has abnormal motility.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

McVittie A (1972) Genetic studies on flagellum mutants of Chlamydomonas reinhardtii. Genet Res 19:157-164

From Pierre Bennoun, Institut de Biologie Physico-Chimique, January 1980

Phenotype: requires acetate

This is a mutation in the chloroplast genome that produces deficiency in chloroplast ATP synthase activity. It was isolated by Bennoun after treatment of cells with 5-fluorodeoxyuridine. The gene affected has not been determined.

Bennoun P, Masson A, Piccioni R, Chua NH (1978) Uniparental mutants of Chlamydomonas reinhardi defective in photosynthesis. Dev Plant Biol 721-726

  • Locus:
  • Chromosome:
  • chloroplast

From Pierre Bennoun, Institut de Biologie Physico-Chimique, January 1980

Phenotype: requires acetate

This is a mutation in the chloroplast genome that produces deficiency in chloroplast ATP synthase activity. It was isolated by Bennoun after treatment of cells with 5-fluorodeoxyuridine. The gene affected has not been determined.

Bennoun P, Masson A, Piccioni R, Chua NH (1978) Uniparental mutants of Chlamydomonas reinhardi defective in photosynthesis. Dev Plant Biol 721-726

  • Locus:
  • Chromosome:
  • chloroplast

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using acridine orange as mutagen. It does not swim.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

McVittie A (1972) Genetic studies on flagellum mutants of Chlamydomonas reinhardtii. Gen Res 19:157-164

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: wall deficient

This is one of the original cell wall deficient mutants isolated by Davies and colleagues, in the 137c background. Davies included cw17 in his class A: cell walls are produced in more or less normal amounts, but are not attached to the plasmalemma.

Like other wall-deficient mutants, cw17 forms flat, liquid colonies on agar, and the cells lyse in a 1% solution of the nonionic detergent NP-40.

Hyams J, Davies DR (1972) The induction and characterization of cell wall mutants of Chlamydomonas reinhardi. Mut Res 14:381-389

Voigt J, Hinkelmann B, Harris EH (1997) Production of cell wall polypeptides by different cell wall mutants of the unicellular green alga Chlamydomonas reinhardtii. Microbiol Res 152:189-198

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. Motility was noted as impaired, but it was not characterized further.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It has short flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using hydroxylamine as mutagen. It was reported to have impaired motility but was not characterized further. In our tests, CC-826 swam slowly.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has impaired motility but was not characterized further. In our tests, CC-827 swam slowly.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen, and was originally named NG5. It has long flagella, and is now known as lf1-1. CC-802 is the same mutant, and is the one that we normally distribute since it has been more thoroughly characterized.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

  • Locus:
  • LF1
  • Chromosome:
  • 2

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It lacks flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It was reported to have impaired motility but was not characterized further. In our tests CC-831 showed apparently normal swimming, suggesting it has reverted or acquired a second-site suppressor.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. Its flagella were described as swollen.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It lacks flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This mutant was obtained by McVittie in the 137c background, using nitrosoguanidine as mutagen. It has stumpy flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: impaired motility

This is a spontaneous mutant isolated by McVittie in the 137c background. It has short flagella.

McVittie A (1972) Flagellum mutants of Chlamydomonas reinhardii. J Gen Microbiol 71:525-540

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: UV sensitive

This mutation was induced by Davies in the 137c background, using nitrosoguanidine as mutagen. It is UV-sensitive, and slower than wild type at excision of thymidine dimers.

Davies DR, Lyall V (1973) The assembly of a highly ordered component of the cell wall: the role of heritable factors and of physical structure. Mol Gen Genet 124:21-34

Small GD (1987) Repair systems for nuclear and chloroplast DNA in Chlamydomonas reinhardtii. Mut Res 181:31-36

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: UV sensitive

This mutation was induced by Davies in the 137c background, using nitrosoguanidine as mutagen. Small and Greimann found that the uvs1 mutant is blocked in dark excision of thymidine dimers.

This is probably the uvs-1/B isolate of Davies, a minority population of the uvs1 cultures that had a wild-type response to oxygen deprivation, whereas most cells in the population were more sensitive to oxygen deprivation than wild-type cells.

Davies DR, Lyall V (1973) The assembly of a highly ordered component of the cell wall: the role of heritable factors and of physical structure. Mol Gen Genet 124:21-34

Small GD, Greimann CS (1977) Photoreactivation and dark repair of ultraviolet light-induced pyrimidine dimers in chloroplast DNA. Nucleic Acids Res 4:2893-2902

Small GD (1987) Repair systems for nuclear and chloroplast DNA in Chlamydomonas reinhardtii. Mut Res 18:31-36

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: wall deficient

This is one of the original cell wall deficient mutants isolated by Davies and colleagues, in the 137c background. Davies included cw1 in his class A: cell walls are produced in more or less normal amounts, but are not attached to the plasmalemma.

Like other wall-deficient mutants, cw1 forms flat, liquid colonies on agar, and the cells lyse in a 1% solution of the nonionic detergent NP-40.

Hyams J, Davies DR (1972) The induction and characterization of cell wall mutants of Chlamydomonas reinhardi. Mut Res 14:381-389

Voigt J, Hinkelmann B, Harris EH (1997) Production of cell wall polypeptides by different cell wall mutants of the unicellular green alga Chlamydomonas reinhardtii. Microbiol Res 152:189-198

Culture Centre of Algae and Protozoa (CCAP), January 1980

Phenotype: wall deficient

This is one of the original cell wall deficient mutants isolated by Davies and colleagues, in the 137c background. Davies included cw14 in his class A: cell walls are produced in more or less normal amounts, but are not attached to the plasmalemma.

Like other wall-deficient mutants, cw14 forms flat, liquid colonies on agar, and the cells lyse in a 1% solution of the nonionic detergent NP-40.

This mutation may be linked to or allelic with cw51.

Hyams J, Davies DR (1972) The induction and characterization of cell wall mutants of Chlamydomonas reinhardi. Mut Res 14:381-389

Voigt J, Hinkelmann B, Harris EH (1997) Production of cell wall polypeptides by different cell wall mutants of the unicellular green alga Chlamydomonas reinhardtii. Microbiol Res 152:189-198