Strains
From Sean Gallaher, Merchant lab, UCLA, December 2014
Gallaher SD, Fitz-Gibbon ST, Glaesener AG, Pellegrini M, Merchant SS. (2015) Chlamydomonas Genome Resource for Laboratory Strains Reveals a Mosaic of Sequence Variation, Identifies True Strain Histories, and Enables Strain-Specific Studies. Plant Cell 27:2335-52
From Sean Gallaher, Merchant lab, UCLA, December 2014
Gallaher SD, Fitz-Gibbon ST, Glaesener AG, Pellegrini M, Merchant SS. (2015) Chlamydomonas Genome Resource for Laboratory Strains Reveals a Mosaic of Sequence Variation, Identifies True Strain Histories, and Enables Strain-Specific Studies. Plant Cell 27:2335-52
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, March 2015
CC-5113 nphp4 mt+ [79D8]
$30.00
$30.00
From Junya Awata, Witman lab, University of Massachusetts Medical School, February 2015
The nphp4/drc3 strain, B1179 (nphp4-1, drc3-1, arg7, ARG7, mt+) was generated by insertional mutagenesis of H11 (arg7, mt+) with the pARG7.8 plasmid and crossed sequentially to the wild-type strains CC-124 and 137c mt+ (Witman lab strain, probably of same origin as CC-125). 79D8 was created by transformation of the crossed B1179 with DRC3.
Mutant cell lacks entire NPHP4 and has defects in swimming path and flagellar regeneration rate.
Awata J, Takada S, Standley C, Lechtreck KF, Bellvé KD, Pazour GJ, Fogarty KE, Witman GB (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci. 127:4714-27
CC-5114 NPHP4-R mt+ [C9]
$30.00
$30.00
From Junya Awata, Witman lab, University of Massachusetts Medical School, February 2015
Phenotype rescue of CC-5113 nphp4 mt+ [79D8] by transformation with NPHP4.
Awata J, Takada S, Standley C, Lechtreck KF, Bellvé KD, Pazour GJ, Fogarty KE, Witman GB (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci. 127:4714-27
From Junya Awata, Witman lab, University of Massachusetts Medical School, February 2015
Transformation of nphp4/drc3 strain, B1179 (nphp4-1, drc3-1, arg7, ARG7, mt+) with NPHP4-HAC (gene encoding nephrocystin-4 tagged with 3xHA peptides on its C-terminus) followed by two crosses to wild-type strains CC-124 and 137c mt+ (Witman lab strain, probably of same origin as CC-125) and transformation of selected offspring with DRC3.
Awata J, Takada S, Standley C, Lechtreck KF, Bellvé KD, Pazour GJ, Fogarty KE, Witman GB (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci. 127:4714-27
CC-5116 NPHP4-HAN mt+ [2C11]
$30.00
$30.00
From Junya Awata, Witman lab, University of Massachusetts Medical School, February 2015
Transformation of nphp4 (79D8) with NPHP4-HAN (gene encoding nephrocystin-4 tagged with 3xHA peptides on its N-terminus).
Awata J, Takada S, Standley C, Lechtreck KF, Bellvé KD, Pazour GJ, Fogarty KE, Witman GB (2014) NPHP4 controls ciliary trafficking of membrane proteins and large soluble proteins at the transition zone. J Cell Sci. 127:4714-27
From Tyler Wittkopp, Grossman lab, Carnegie Institution of Washington-Stanford, April 2015
The Grossman lab strain 21gr- was created the following way:
1) 21gr nit+ mt+ was crossed to psr1 nit2 mt- (Moseley et al.)
2) 21gr nit+ mt- progeny were selected and backcrossed 3 times to 21gr mt+ to get the final strain
Moseley JL, Gonzalez-Ballester D, Pootakham W, Bailey S, Grossman AR (2009) Genetic interactions between regulators of Chlamydomonas phosphorus and sulfur deprivation responses. Genetics 181: 889-905
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This strain was derived from 4 backcrosses of CC-645 (pab2 mt-) to an arg 7-8 mt+ strain in the Niyogi lab.
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This strain was derived from 4 backcrosses of CC-645 (pab2 mt-) to an arg 7-8 mt+ strain in the Niyogi lab.
CC-5122 GPXH-OE A1-5 mt+
$30.00
$30.00
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This mutant was created by transforming CC-4051 4A+ with the ProPSAD:GPXH plasmid which resulted in constitutive overexpression of GPXH.
Ledford HK, Chin BL, Niyogi KK (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6: 919–930.
CC-5123 GPXH-OE A2-5 mt+
$30.00
$30.00
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This mutant was created by transforming CC-4051 4A+ with the ProPSAD:GPXH plasmid which resulted in constitutive overexpression of GPXH.
Ledford HK, Chin BL, Niyogi KK (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6: 919–930.
CC-5124 GPXH-OE C2-3 mt+
$30.00
$30.00
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This mutant was created by transforming CC-4051 4A+ with the ProPSAD:GPXH plasmid which resulted in constitutive overexpression of GPXH.
Ledford HK, Chin BL, Niyogi KK (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6: 919–930.
CC-5125 GSTS1-OE A2-7 mt+
$30.00
$30.00
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This mutant was created by transforming CC-4051 4A+ with the ProPSAD:GSTS1 plasmid which resulted in constitutive overexpression of GSTS1.
Ledford HK, Chin BL, Niyogi KK (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6: 919–930.
CC-5126 GSTS1-OE B1-7 mt+
$30.00
$30.00
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This mutant was created by transforming CC-4051 4A+ with the ProPSAD:GSTS1 plasmid which resulted in constitutive overexpression of GSTS1.
Ledford HK, Chin BL, Niyogi KK (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6: 919–930.
CC-5127 GSTS1-OE B2-6 mt+
$30.00
$30.00
From Marilyn Kobayashi, Niyogi lab, University of California-Berkeley, April 2015
This mutant was created by transforming CC-4051 4A+ with the ProPSAD:GSTS1 plasmid which resulted in constitutive overexpression of GSTS1.
Ledford HK, Chin BL, Niyogi KK (2007) Acclimation to singlet oxygen stress in Chlamydomonas reinhardtii. Eukaryot Cell 6: 919–930.
CC-5128 hydEF-1 mt+
$30.00
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation of the iron hydrogenase assembly protein by insertion of pJD67 into CC-425.
HYDEF/HYDEF1: chromosome_06: 7003406..7012157
Posewitz MC, King PW, Smolinski SL, Zhang L, Seibert M, Ghirardi ML (2004) Discovery of two novel radical S-adenosylmethionine proteins required for the assembly of an active [Fe] hydrogenase. J Biol Chem. 279:25711-20
CC-5129 sta7 mt+
$30.00
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation of CC-425 by insertion of pJD67, which contains a functional copy of the genomic argininosuccinate lyase (Arg7) gene.
STA7: chromosome_3: 1885221..1896321
Posewitz MC, Smolinski SL, Kanakagiri S, Melis A, Seibert M, Ghirardi ML (2004) Hydrogen photoproduction is attenuated by disruption of an isoamylase gene in Chlamydomonas reinhardtii. Plant Cell 16:2151–2163
CC-5130 hydG hydA2-1 mt+
$30.00
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation in HYDA2 (Iron hydrogenase) by insertion of pSL72 into D66 (CC-4425 cw15 nit2-203 mt+), carrying AphVIII gene (paromomycin resistance); mutation in HYDG (Hydrogenase assembly factor/biotin synthase) by insertion of pSP124S plasmid, carrying Zeo R gene (Zeocin).
HYDG: chromosome_6:6998683..7003289
HYDA2: chromosome_9:1740957..1745328
CC-5131 hydA1-1 hydA2-1 mt+
$30.00
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation in HYDA2 (Iron hydrogenase) by insertion of pSL72 carrying AphVIII gene (paromomycin resistance); mutation in HYDA1 (Iron hydrogenase) by insertion of pSP124S carrying Zeo R gene (Zeocin). Crossed 4X with D66 (CC-4425 cw15 nit2-203 mt+) and CC-124.
HYDA1: chromosome_3:8216108..822075
HYDA2: chromosome_9:1740957..1745328
Meuser JE, D'Adamo S, Jinkerson RE, Mus F, Yang W, Ghirardi ML, Seibert M, Grossman AR, Posewitz MC (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H₂ production. Biochem Biophys Res Commun. 417:704-9
CC-5132 hydA1-1 mt+
$30.00
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation in HYDA1 (Iron hydrogenase) by insertion of pSP124S carrying Zeo R gene (Zeocin). Crossed 4X with D66 (CC-4425 cw15 nit2-203 mt+) and CC-124.
HYDA1: chromosome_3:8216108..822075
Meuser JE, D'Adamo S, Jinkerson RE, Mus F, Yang W, Ghirardi ML, Seibert M, Grossman AR, Posewitz MC (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H₂ production. Biochem Biophys Res Commun. 417:704-9
CC-5133 hydA2-1 mt+
$30.00
$30.00
From Sarah D’Adamo, Matthew Posewitz lab, Colorado School of Mines, Golden CO, July 2015
Mutation in HYDA2 by insertion of pSL72 plasmid, carrying AphVIII gene (paromomycin resistance). Crossed 4X with D66 (CC-4425 cw15 nit2-203 mt+) and CC-124.
HYDA2: chromosome_9:1740957..1745328
Meuser JE, D'Adamo S, Jinkerson RE, Mus F, Yang W, Ghirardi ML, Seibert M, Grossman AR, Posewitz MC (2012) Genetic disruption of both Chlamydomonas reinhardtii [FeFe]-hydrogenases: Insight into the role of HYDA2 in H₂ production. Biochem Biophys Res Commun. 417:704-9
From David Polley, Wabash College, July 2015
Phenotype: requires high potassium concentration
This strain requires high potassium (concentrations greater than 1mM) for growth. Wild-type growth rates require 10mM K. Use agarose solidified medium when testing for phenotype on plate cultures.
This mutant was UV induced in a trk2-1 act1 recombinant produced from a cross between CC-1715 (pf18 nic2 act1 mt-) and CC-3832. Phenotype expression requires trk2-1 background, but phenotype expression in trk3 or trk1 background has not been tested.
Polley LD (1999) Genetic analysis of mutant clones of Chlamydomonas reinhardtii defective in potassium transport. Mol. Gen. Genet. 261, 275-280
CC-5135 trk2-2 mt+ [KDP2]
$30.00
$30.00
From David Polley, Wabash College, July 2015
Phenotype: requires high potassium concentration
This strain requires potassium concentrations greater than 0.1mM for growth. Use agarose solidified medium when testing for phenotype on plate cultures. Wild-type growth rates require 1mM K. Exhibits altered transport kinetics.
This mutant was UV-induced by Polley and Doctor in a strain equivalent to CC-620 (137c background).
Polley LD & Doctor DD (1985) Potassium transport in Chlamydomonas reinhardtii: isolation and characterization of transport-deficient mutant strains. Planta 163, 208-213
Polley LD (1999) Genetic analysis of mutant clones of Chlamydomonas reinhardtii defective in potassium transport. Mol. Gen. Genet. 261, 275-280
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