Strains

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated from a cross between kif6 smg1-2 and dgr14-1 to remove the kif6 mutation. There is no apparent growth or motility defect.

smg1-2: Mis-sense mutation at chromosome_13:1417593 (G > T)
dgr14-1: 33 kb deletion on chromosome_11: 3603615 – 3636297

Lin H, Zhang Z, Iomini C, Dutcher SK (2018) Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii. Open Biol. vol.8(3)

  • Locus:
  • SMG1, DGR14
  • Chromosome:
  • 13, 11

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated from a cross between kif6 smg1-2 and fra10-1 to remove the kif6 mutation. There is no apparent growth or motility defect.

smg1-2: Mis-sense mutation at chromosome_13:1417593 (G > T)
fra10-1: Deletion at chromosome_7:3538004 (deletion of C)

Lin H, Zhang Z, Iomini C, Dutcher SK (2018) Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii. Open Biol. vol.8(3)

  • Locus:
  • SMG1, FRA10
  • Chromosome:
  • 13, 7

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

The origin of this strain is from a spontaneous mutation found in the strain CC-4348, which contains multiple mutations on various genes. The fla11-2 mutant is aflagellate.

An insertion into the exon 2 of IFT172 at chromosome_17: 1081293

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

The mutant phenotype of fla11-2 was rescued by introducing the transgene through a cross between fla11-2 and IFT172-FLAG.

Notes:
Wild-type IFT172 tagged with a 3xFLAG tag at the N-terminus
The tagged plasmid was transformed into wild-type cells

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by a cross between fla11-2 IFT172-FLAG and CC-5116 (NPHP4-HAN). This strain has normal flagellar assembly.

Notes:
Wild-type IFT172 tagged with a 3xFLAG tag at the N-terminus

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by a cross between fla11-2 IFT172-FLAG and CC-5116 (NPHP4-HAN). This strain is aflagellate.

Notes:
Wild-type IFT172 tagged with a 3xFLAG tag at the N-terminus

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

Generated by a cross between ift121-2 and NPHP4-HAN. This strain is aflagellate.

ift121-2: point mutation in the IFT121 gene, affects a splice site (c.2754+1 G>A) at chromosome_11:2411434

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

  • Locus:
  • IFT121
  • Chromosome:
  • 11

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by a UV mutagenesis screen to identify aflagellate mutants. This strain is aflagellate.

Notes:
A nonsense mutation in the RPGRIP1L gene at chromosome_14: 3725856 that leads to K629Stop

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by a cross between rpg1-1 and NPHP4-HAN. This strain is aflagellate.

Notes:
A nonsense mutation in the RPGRIP1L gene at chromosome_14: 3725856 that leads to K629Stop

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

The ift140-3 mutant was generated by UV mutagenesis screen to identify aflagellate mutants. This strain is generated by a cross between ift140-3 and NPHP4-HAN. This strain is aflagellate.

Notes:
A nonsense mutation in the IFT140 gene at chromosome_8: 1134675-76 that leads to K386Stop

Lin H, Guo S, Dutcher SK (2018) RPGRIP1L helps to establish the ciliary gate for entry of proteins. J Cell Sci. Oct 26;131(20)

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and is aflagellate.

Insertion into exon 18 of FLA10 (Cre17.g730950) at chromosome_17: 4322754-4322816

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • FLA10
  • Chromosome:
  • 17

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious phenotype.

Insertion into intron 9 of MYSM1 (Cre03.g176651) at chromosome_3: 4539389-4539406

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • MYSM1
  • Chromosome:
  • 3

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious phenotype.

Insertion into 3’ UTR of FAP22 (Cre17.g721250) at chromosome_17: 3105419-3105428

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • FAP22
  • Chromosome:
  • 17

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and is aflagellate and contains complex DNA rearragement.

Insertion/deletion into multiple genes: deletion of 841 bp (exons 5-8) on Cre02.g145950 at chromosome_2: 7226958-7227798; insertion into 3’ UTR of PTK24 at chromosome_2: 7290053; an unknown region starting at chromosome_12: 7660573 that has no predicted gene is likely to insert into chromosome_2.

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious phenotype. This is the only mutation identified by whole genome sequencing but no cosegregation test has been done.

Insertion into the TPRC3 (Cre01.g029400) gene at chromosome_1: 4336001-4336135

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • TPRC3
  • Chromosome:
  • 1

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and is aflagellate.

Insertion into exon 13 of the BLD11 (Cre16.g672200) gene at chromosome_16:6582926-6582930

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and is aflagellate.

Insertion into intron 14 and exon 15 of the BLD11 (Cre16.g672200) gene at chromosome_16: 6583673-6583708

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype.

Insertion into 5’ UTR of TMEM45B (Cre06.g283900) at chromosome_6: 5398524-5398526

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype.

Insertion into 5’ UTR of Cre02.g086300 at chromosome_2: 1736814

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype.

Insertion into exon 4 of Cre06.g278262 at chromosome_6: 4339867-4339917

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype but contains complex DNA rearragement.

Insertion into 5’ UTR of Cre06.g278107 at chromosome_6: 3512412, a piece from chromosome_5: 1676418 is also inserted into this region

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has a slow growth defect.

Insertion into intron 16 of MITC17 (Cre16.g650200) at chromosome_16: 1141900

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • MITC17
  • Chromosome:
  • 16

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype.

Insertion into 3’ UTR of ATG11 (Cre16.g651350) at chromosome_16:1266125-1266130

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • ATG11
  • Chromosome:
  • 16

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype.

Insertion into exon 13 of Cre06.g263650 at chromosome_6: 1938823-1938849

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype but contains complex DNA rearrangement.

Insertion into intron 11 of PHC10 (Cre09.g404201) at chromosome_9: 497660, with DNA fragments from chromosome_13: 3741836

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • PHC10
  • Chromosome:
  • 9

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and contains multiple flagella.

Insertion into intron 5 of the BAR1 (Cre16.g653450) gene at chromosome_16: 1549854-1549855

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

  • Locus:
  • BAR1
  • Chromosome:
  • 16

From Huawen Lin, Susan Dutcher lab, Washington University in St. Louis, February 2019

This strain was generated by insertional mutagenesis and has no obvious mutant phenotype.

Insertions into 5’ UTR of Cre17.g735550 at chromosome_17: 5178817 and exon 5 of Cre17.g736000 at chromosome_17: 5244723

Lin H, Cliften PF, Dutcher SK (2018) MAPINS, a Highly Efficient Detection Method That Identifies Insertional Mutations and Complex DNA Rearrangements. Plant Physiol. 178(4):1436-1447

From Tyler Picariello, George Witman lab, University of Massachusetts Medical School, February 2019

Picariello T, Brown JM, Hou Y, Swank G, Cochran DA, King OD, Lechtreck K, Pazour GJ, Witman GB (2019) A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia. J Cell Sci. Feb 11;132(3)

  • Locus:
  • IFT140
  • Chromosome:
  • 8

From Tyler Picariello, George Witman lab, University of Massachusetts Medical School, February 2019

Picariello T, Brown JM, Hou Y, Swank G, Cochran DA, King OD, Lechtreck K, Pazour GJ, Witman GB (2019) A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia. J Cell Sci. Feb 11;132(3)

  • Locus:
  • IFT140
  • Chromosome:
  • 8

From Tyler Picariello, George Witman lab, University of Massachusetts Medical School, February 2019

Picariello T, Brown JM, Hou Y, Swank G, Cochran DA, King OD, Lechtreck K, Pazour GJ, Witman GB (2019) A global analysis of IFT-A function reveals specialization for transport of membrane-associated proteins into cilia. J Cell Sci. Feb 11;132(3)

  • Locus:
  • IFT140
  • Chromosome:
  • 8