From Lei Zhao and George Witman, University of Massachusetts Medical School, August 2019

This strain was generated by cross of LMJ.RY0402.135524 to wild-type strain g1 (CC-5415).


Zhao L, Hou YQ, Picariello T, Craige B, Witman GB (2019) Proteome of the central apparatus of a ciliary axoneme. J Cell Biol. 218: 2051-2070


  • Locus:
  • FAP246
  • Chromosome:
  • 14

From Lei Zhao and George Witman, University of Massachusetts Medical School, August 2019

This strain was generated by transformation of fap246-1 with the plasmid pBC22 containing wild-type FAP246 genomic sequence with a 3xHA tag; this plasmid also contains a hygromycin-resistance cassette (Berthold et al., 2002, Protist).


Zhao L, Hou YQ, Picariello T, Craige B, Witman GB (2019) Proteome of the central apparatus of a ciliary axoneme. J Cell Biol. 218: 2051-2070


  • Locus:
  • FAP246
  • Chromosome:
  • 14

From Lei Zhao and George Witman, University of Massachusetts Medical School, August 2019

This strain was generated by cross of LMJ.RY0402.074963 to wild-type strain g1 (CC-5415).


Zhao L, Hou YQ, Picariello T, Craige B, Witman GB (2019) Proteome of the central apparatus of a ciliary axoneme. J Cell Biol. 218: 2051-2070


  • Locus:
  • DPY30
  • Chromosome:
  • 6

From Lei Zhao and George Witman, University of Massachusetts Medical School, August 2019

This strain was generated by transformation of dpy30-1 with the plasmid pBC24 containing wild-type DPY30 genomic sequence with a 3xHA tag; this plasmid also contains a hygromycin-resistance cassette (Berthold et al., 2002, Protist).


Zhao L, Hou YQ, Picariello T, Craige B, Witman GB (2019) Proteome of the central apparatus of a ciliary axoneme. J Cell Biol. 218: 2051-2070


  • Locus:
  • DPY30
  • Chromosome:
  • 6