From Thamali Kariyawasam and Jae-Hyeok Lee, University of British Columbia, December 2019


Kariyawasam T, Joo S, Lee J, Toor D, Gao AF, Noh KC, Lee JH (2019) TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas. Plant J. 100:938-953

From Thamali Kariyawasam and Jae-Hyeok Lee, University of British Columbia, December 20199


Kariyawasam T, Joo S, Lee J, Toor D, Gao AF, Noh KC, Lee JH (2019) TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas. Plant J. 100:938-953

Lee JH, Lin H, Joo S, Goodenough U (2008) Early sexual origins of homeoprotein heterodimerization and evolution of the plant KNOX/BELL family. Cell. 133:829-40

From Thamali Kariyawasam and Jae-Hyeok Lee, University of British Columbia, December 2019


Kariyawasam T, Joo S, Lee J, Toor D, Gao AF, Noh KC, Lee JH (2019) TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas. Plant J. 100:938-953

Lee JH, Lin H, Joo S, Goodenough U (2008) Early sexual origins of homeoprotein heterodimerization and evolution of the plant KNOX/BELL family. Cell. 133:829-40

From Evan Cronmiller and Jae-Hyeok Lee, University of British Columbia. December 2019

This strain has high mating and transformation efficiency. It is the progeny of CC-3491 (cw15 mt-) and CC-85 (nic7 mt+), isolate 1926.


Cronmiller E, Toor D, Shao NC, Kariyawasam T, Wang MH, Lee JH (2019) Cell wall integrity signaling regulates cell wall-related gene expression in Chlamydomonas reinhardtii. Scientific reports, 9(1), 12204. doi:10.1038/s41598-019-48523-4


  • Locus:
  • NIC7 [QSA1]
  • Chromosome:
  • 6

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8 

This strain originally came from Dr. Jean David Rochaix (University of Geneva) and is a cross of CC-124 with an arg7-8 mutant strain.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8 

This strain is originally from Dr. Jean David Rochaix (University of Geneva) and is a cross of CC-124 with an arg7-8 mutant strain.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and was created by mutagenesis of 3A+ (CC-5589) with a cassette containing the APHVIII transgene from pSL18. It is a mitochondrial complex I-deficient strain that harbors an insertional mutation in the NUOB10 gene encoding the NUOB10 / PDSW subunit of mitochondrial complex I. It is paromomycin resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10; ARG7

This is the amc5 strain complemented with the wild-type NUOB10 gene.

Mutagenesis of amc5 with cosmid 7D10 (Rochaix cosmid library) containing the wild-type NUOB10 gene and selected based on ARG7 marker in the cosmid. It is paromomycin resistant and an arginine prototroph.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10-FLAG; APHVII

This is the amc5 strain complemented with the wild-type NUOB10-FLAG gene.

Mutagenesis of amc5 with cosmid plasmid pRS426 containing the wild-type NUOB10 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C79S-FLAG; APHVII

This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C79S substitution.

Mutagenesis of amc5 with cosmid plasmid pRS426 containing the wild-type NUOB10 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C91S-FLAG; APHVII

This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C91S substitution.

Mutagenesis of amc5 with cosmid plasmid pRS426 containing the NUOB10 gene encoding the C91S substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Yes


From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; nuob10::APHVIII; NUOB10C79S,C91S-FLAG; APHVII

This is the amc5 strain complemented with the NUOB10-FLAG gene encoding C79S and C91S substitution.

Mutagenesis of amc5 with plasmid pRS426 containing the NUOB10 gene encoding the double C79S and C91S substitutions with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


  • Locus:
  • Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.
  • Chromosome:

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; amc1-1; APHVII

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is mitochondrial complex I-deficient. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. APHVII transgene present in an unknown location. This strain in hygromycin resistant.

It was created by mutagenesis of 3A+ with a cassette containing the APHVII transgene from pHyg3.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc1-1

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is mitochondrial complex I-deficient. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. It is a cross of amc1(4C10) with CC-124 resulting in meiotic progeny that is arg+ and hygromycin sensitive.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1). Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin sensitive and an arginine prototroph.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-1

This strain is originally from Rosario Barbieri/Patrice P. Hamel (OSU) and is a cross of amc1(4C10) with 4C- resulting in meiotic progeny that is arg- and hygromycin sensitive. Genomic DNA from chromosome 17 disrupts Cre16.g688900 in exon 2. APHVII transgene present in an unknown location. Originally isolated as a mitochondrial complex I-deficient strain but now the phenotype has reverted.

This strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin sensitive.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII; amc8

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain is hygromycin resistant.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. The APHVII transgene disrupts exon 2 of NUO5 gene Cre10.g450400. It is a mitochondrial complex I-deficient strain and is hygromycin resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5; ARG7

This is the amc9 strain complemented with the wild-type Cre10.g450400 gene. It was created by mutagenesis of amc9 with cosmid 9A2 (Rochaix cosmid library) containing the full-length wild-type NUO5 (Cre10.g450400) and selected based on ARG7 marker in the cosmid. This strain is hygromycin resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5-FLAG; APHVIII

This is the amc9 strain complemented with the wild-type NUO5 gene with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the wild-type NUO5 gene with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; nuo5::APHVII; NUO5K230R-FLAG; APHVIII

This is the amc9 strain complemented with the NUO5 gene containing the K230R substitution with a C-terminal FLAG tag. It was created by mutagenesis of amc9 with plasmid pRS426 containing the NUO5 gene encoding the K230R substitution with a C-terminal FLAG tag and APHVII selection marker. It is paromomycin and hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII; amc10

This strain was originally a mitochondrial complex I-deficient strain that reverted in its phenotype. It was originally created by mutagenesis of 4C- with a cassette containing APHVII transgene from pHyg3. It is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; APHVII; amc10

This strain is meiotic progeny from a cross of amc10 (42A10) with CC-124. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII; amc10

This strain is meiotic progeny from a cross of amc10 (42A10) with CC-124. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

Yes


From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2;APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


  • Locus:
  • Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.<br><br><br>Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:

Yes


From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2; APHVII; AMC1; ARG7

This is the amc11 strain complemented with the wild-type Cre16.g688900 gene.

This strain was created by mutagenesis of amc11 with cosmid 1H10 (Rochaix cosmid library) containing the full-length wild-type AMC11 (Cre16.g688900) and CYG56 (Cre16.g688901) genes and selected based on ARG7 marker in the cosmid. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).


  • Locus:
  • Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:

Yes


From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; amc1-2; APHVII; AMC1; ARG7

This is the amc11 strain complemented with the wild-type Cre16.g688900 gene.

This strain was created by mutagenesis of amc11 with truncated cosmid 1H10 (Rochaix cosmid library) containing the wild-type AMC11 (Cre16.g688900) gene and selected based on ARG7 marker in the cosmid. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1).


  • Locus:
  • Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.
  • Chromosome:

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVI; amc13

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. It is a mitochondrial complex I-deficient strain and is hygromycin B resistant.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc13

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. It is a mitochondrial complex I-deficient strain and is hygromycin B sensitive and an arginine auxotroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; amc1-2; APHVII

This is a mitochondrial complex I-deficient strain. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is hygromycin B resistant and an arginine prototroph.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1). Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.