From Heide Evers, Hegemann lab, Humboldt University-Berlin, January 2020

Strain PH239 ∆COP1-F6 includes the 581 bp insert inside COP1

From Shan He, Martin Jonikas Lab, Department of Molecular Biology, Princeton University, February 2020

Maintain in low light (~50 μmol photons m-2 s-1), in Tris-Acetate-Phosphate medium or in Tris-Phosphate medium at 3% v/v CO2.


  • Locus:
  • RBCS2
  • Chromosome:
  • 2

From Shan He, Martin Jonikas Lab, Department of Molecular Biology, Princeton University, February 2020

Maintain in low light (~50 μmol photons m-2 s-1), in Tris-Acetate-Phosphate medium or in Tris-Phosphate medium at 3% v/v CO2.


  • Locus:
  • RBCS2
  • Chromosome:
  • 2

From Shan He, Martin Jonikas Lab, Department of Molecular Biology, Princeton University, February 2020

Maintain in low light (~50 μmol photons m-2 s-1), in Tris-Acetate-Phosphate medium or in Tris-Phosphate medium at 3% v/v CO2.


  • Locus:
  • RBCS2
  • Chromosome:
  • 2

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

DNA insertional mutant CAL028_03_28 from the CAL collection which is defective in the RBD1 gene (Cre07.g315150).

Maintain on TAP plates containing 25 μg/mL paromomycin in dark or very low light.


Calderon RH, Garc’a-Cerd‡n JG, Malno‘ A, Cook R, Russell JJ, Gaw C, Dent RM, de Vitry C, Niyogi KK (2013) A conserved rubredoxin is necessary for photosystem II accumulation in diverse oxygenic photoautotrophs. J Biol Chem. 288:26688-96

García-Cerdán JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640


  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

The 2pac mutant CAL028_03_28 was transformed with WT RBD1 cDNA under control of PSAD promoter and terminator. Constitutive overexpression of WT RBD1.

Maintain on TAP plates containing 25 μg/mL hygromycin and/or paromomycin.


Garc’a-Cerd‡n JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640


  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

The 2pac mutant CAL028_03_28 was transformed with mutant RBD1 cDNA under control of PSAD promoter and terminator. Constitutive overexpression of C76/109G mutant version of RBD1.

Maintain on TAP plates containing 25 μg/mL hygromycin and/or paromomycin.


Garc’a-Cerd‡n JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640


  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

The 2pac mutant CAL028_03_28 was transformed with mutant RBD1 cDNA under control of PSAD promoter and terminator. Constitutive overexpression of ΔTMH mutant version of RBD1

Maintain on TAP plates containing 25 μg/mL hygromycin and/or paromomycin.


Garc’a-Cerd‡n JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640


  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Dr. Krishna Niyogi, University of California-Berkeley, February 2020

The 2pac mutant CAL028_03_28 was transformed with mutant RBD1 cDNA under control of PSAD promoter and terminator. Constitutive overexpression of ΔTP mutant version of RBD1

Maintain on TAP plates containing 25 μg/mL hygromycin and/or paromomycin.


Garc’a-Cerd‡n JG, Furst AL, McDonald KL, Schünemann D, Francis MB, Niyogi KK (2019) A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II. Proc Natl Acad Sci U S A. 116:16631-16640


  • Locus:
  • RBD1
  • Chromosome:
  • 7

From Rosario Barbieri, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3. This strain in hygromycin resistant.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

From Rosario Barbieri, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; arg7-8; APHVII

This strain was created by mutagenesis of 4C- with a cassette containing the APHVII transgene from pHyg3.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain in hygromycin resistant.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

From Rosario Barbieri, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; APHVIII

This strain was created by mutagenesis of 3A+ with a cassette containing the APHVII transgene from pSL18.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain in paromomycin resistant.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

From Rosario Barbieri, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; arg7-8; APHVIII

This strain was created by mutagenesis of 3A+ with a cassette containing the APHVII transgene from pSL18.

It should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient. This strain in paromomycin resistant.


Barbieri MR, Larosa V, Nouet C, Subrahmanian N, Remacle C, Hamel PP. A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii. Genetics. 2011 Jun;188(2):349-58. doi: 10.1534/genetics.111.128827. Epub 2011 Apr 5. PubMed PMID: 21467570; PubMed Central PMCID: PMC3122308.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; amc13

This strain is a genetic cross of amc13 (4C3) and CC-125.

This mitochondrial complex I-deficient strain should be maintained in low-medium light conditions (max. 50 µmol.m-2.s-1) and the media should contain arginine. Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Fatnes TA, Hamel PP. Chlamydomonas reinhardtii as a plant model system to study mitochondrial complex I dysfunction. Plant Direct. 2020 Feb 3;4(2):e00200. doi: 10.1002/pld3.200. eCollection 2020 Feb. PubMed PMID: 32025618; PubMed Central PMCID: PMC6996877.

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc1-2; APHVII

This mitochondrial complex I-deficient strain is a genetic cross of amc11(10G11) with a 137c mt+ strain (1′) from Dr. Claire Remacle (University of Liege) selected for arg+, hygromycin B resistant meiotic prorgeny. The APHVII transgene disrupts Cre16.g688900 in exon 2. It is hygromycin B resistant and an arginine prototroph.

Maintain in medium light conditions (max. 50 µmol.m-2.s-1). Do not maintain in the dark as it is respiratory-deficient.


Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.


  • Locus:
  • AMC1
  • Chromosome:
  • 16

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt-; amc1-2; APHVII; arg9-2

This mitochondrial complex I-deficient strain is a genetic cross of amc11(70) with a complex I proficient derivative of arg9-2 (Hamel strain 141) to select for arg-, hygromycin B resistant meiotic progeny. The APHVII transgene disrupts Cre16.g688900 in exon 2.

Maintain in medium light conditions (max. 50 µmol.m-2.s-1) and not in the dark as it is respiratory-deficient. Media should contain arginine.


Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.


  • Locus:
  • AMC1
  • Chromosome:
  • 16

From Nitya Subrahmanian, Patrice P. Hamel lab, The Ohio State University, February 2020

Genotype: mt+; amc1-2; APHVII; arg9-2

This mitochondrial complex I-deficient strain is a genetic cross of amc11(70) with a complex I proficient derivative of arg9-2 (Hamel strain 141) to select for arg-, hygromycin B resistant meiotic progeny. The APHVII transgene disrupts Cre16.g688900 in exon 2.

Maintain in medium light conditions (max. 50 µmol.m-2.s-1) and not in the dark as it is respiratory-deficient. Media should contain arginine.


Subrahmanian N, Castonguay AD, Remacle C, Hamel PP. Assembly of Mitochondrial Complex I Requires the Low-Complexity Protein AMC1 in Chlamydomonas reinhardtii. Genetics. 2020 Feb 19. pii: genetics.303029.2020. doi: 10.1534/genetics.120.303029. [Epub ahead of print] PubMed PMID: 32075865.


  • Locus:
  • AMC1
  • Chromosome:
  • 16

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Heide Evers, Peter Hegemann lab, Humboldt University-Berlin, July 2020

From Moritz Meyer, Martin Jonikas lab, Princeton University, August 2020

This strain was created by transforming CMJ030 with pLM164 and is paromomycin resistant.

pLM164: pLM099-Cre06.g261750-Venus-3xFLAG

RBMP1 (Cre06.g261750) gDNA coding sequence plus 3672 bp upstream of the start codon was cloned into pLM099 by recombineering to produce a C-terminal CrVenus-3xFLAG tag.


https://www.biorxiv.org/content/10.1101/2020.08.16.252858v1.full

From Moritz Meyer, Martin Jonikas lab, Princeton University, August 2020

This strain was created by transforming CMJ030 with pLM155 and is paromomycin resistant.

pLM155: pLM099-Cre09.g416850-Venus-3xFLAG

RBMP2 (Cre09.g416850) coding sequence plus 2250 bp upstream of the start codon was cloned into pLM099 by recombineering to produce a C-terminal CrVenus-3xFLAG tag.


https://www.biorxiv.org/content/10.1101/2020.08.16.252858v1.full

From Moritz Meyer, Martin Jonikas lab, Princeton University, August 2020

This strain was created by transforming CMJ030 with pMJ503 and is paromomycin resistant.

pMJ503: pLM005-Cre14.g626700-Venus-3xFLAG-3xCtermSAGA2

FDX1 (Cre14.g626700) gDNA coding sequence, spanning the start codon to the triplet just before the stop codon, had been previously cloned in frame into pLM005 by Gibson assembly (as described in https://doi.org/10.1073/pnas.1522866113; CSI_FC1F06 = pLM005-Cre14.g626700-Venus-3xFLAG). That plasmid was then reengineered by restriction digestion and T4 ligation to add in-frame immediately after the 3xFLAG a synthetic fragment containing three copies of the sequence coding for the 15 C-terminal amino acids of SAGA2 (Cre 09.g394621), interspersed with a short flexible linker (GGGGGS).