From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • LF5, Cre12.g538300
  • Chromosome:
  • 12

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • LF5, Cre12.g538300
  • Chromosome:
  • 12

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • Chromosome:
  • LF5, Cre12.g538300

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • LF5, Cre12.g538300
  • Chromosome:
  • 12

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • LF5, Cre12.g538300
  • Chromosome:
  • 12

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • LF5, Cre12.g538300
  • Chromosome:
  • 12

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 6 and 7 of Hou et al., (2022). The endogenous LF5 allele was tagged with 3HA in front of the stop codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • LF5, Cre12.g538300
  • Chromosome:
  • 12

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From George Witman, University of Massachusetts Medical School, January 2023

Background: CC-5415 nit1 agg1 mt+ [Witman g1]

Origin: This strain was generated by the TIM-tagging method, a direct in situ tagging method based on TIM, targeted insertional mutagenesis.

Comment: This strain was one of the strains shown in Figures 8, 9, and 11 of Hou et al., (2022). The endogenous NAP1L1 allele was tagged with mNeonGreen-3xFLAG after the start codon.


Hou Y, Cheng X, Witman GB. Direct in situ protein tagging in Chlamydomonas reinhardtii utilizing TIM, a method for CRISPR/Cas9-based targeted insertional mutagenesis. PLoS One. 2022 Dec 9;17(12):e0278972. doi: 10.1371/journal.pone.0278972. PMID: 36490276; PMCID: PMC9733891.


  • Locus:
  • NAP1L1, Cre09.g416350
  • Chromosome:
  • 9

From Gui Zhang, Karl Lechtreck lab, University of Georgia, January 2023

From Gui Zhang, Karl Lechtreck lab, University of Georgia, February 2023

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: lacks outer arm dynein light chain LC1; paromomycin-resistant

This mutant was obtained by crossing dlu1-1 (LMJ.RY0402.223569) and wild type CC-125. It is designated as strain 5a in King lab.


Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • DLU1 [DLU1]
  • Chromosome:
  • 2

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: unlike the background strain, it restores LC1 expression and does not require arginine to grow; paromomycin-resistant

It was created by transforming mutant dlu1-1 arg7-8 cw15 with pFA-LC1 plasmid containing LC1 and ARG7 expression cassettes in tandem. It is designated as strain R4 in King lab.


Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • DLU1 [DLU1]
  • Chromosome:
  • 2

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: lacks outer arm dynein light chain LC1 and inner arm dynein I1/f; paromomycin-resistant; tends to form palmelloid cells

This mutant was obtained by crossing dlu1-1 (strain 5a, CC-6005) and ida1 (CC-2665). It is designated as strain 6c in King lab.


Kamiya R, Kurimoto E, Muto E. Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein. J Cell Biol. 1991 Feb;112(3):441-7. doi: 10.1083/jcb.112.3.441. PMID: 1825085; PMCID: PMC2288841.

Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • DLU1 [DLU1], IDA1 [DHC1]
  • Chromosome:
  • 2, 12

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: lacks outer arm dynein light chain LC1; reduced axonemal tubulin polyglutamylation; paromomycin-resistant; mixed population of immotile cells and cells showing twitchy motion or swimming

This mutant was obtained by crossing dlu1-1 (strain 5a, CC-6005) and tpg1 (CC-5245).


Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R. Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 2010 Mar 9;20(5):441-5. doi: 10.1016/j.cub.2009.12.058. Epub 2010 Feb 25. PMID: 20188560.

Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • DLU1 [DLU1], TPG1 [FAP267]
  • Chromosome:
  • 2, 17

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: a truncation of the motor domain of outer arm dynein heavy chain γHC

This mutant was obtained by crossing CC-125 and oda2-t ida1 to eliminate an unknown mutation affecting cilia in CC-4439 in addition to oda2-t mutation. Please see Sakato-Antoku and King (2023) for details. It is designated as strain 12c in King lab.


Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008 Jul;7(7):1136-45. doi: 10.1128/EC.00102-08. Epub 2008 May 16. PMID: 18487347; PMCID: PMC2446680.

Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • ODA2 [DHC15]
  • Chromosome:
  • 11

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: a truncation of the motor domain of outer arm dynein heavy chain γHC; lacks inner arm dynein I1/f; tends to form palmelloid cells

This mutant was obtained by crossing oda2-t (strain 12c, CC-6009) and ida1 (CC-2665). It is designated as strain 12c-1b in King lab.


Kamiya R, Kurimoto E, Muto E. Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein. J Cell Biol. 1991 Feb;112(3):441-7. doi: 10.1083/jcb.112.3.441. PMID: 1825085; PMCID: PMC2288841.

Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008 Jul;7(7):1136-45. doi: 10.1128/EC.00102-08. Epub 2008 May 16. PMID: 18487347; PMCID: PMC2446680.

Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • ODA2 [DHC15], IDA1 [DHC1]
  • Chromosome:
  • 11,12

From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023 

Phenotype: a truncation of the motor domain of outer arm dynein heavy chain γHC; reduced axonemal tubulin polyglutamylation; mixed population of immotile cells and cells showing intermittent beating or swimming

This mutant was obtained by crossing oda2-t (strain 12c, CC-6009) and tpg1 (CC-5245). 


Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008 Jul;7(7):1136-45. doi: 10.1128/EC.00102-08. Epub 2008 May 16. PMID: 18487347; PMCID: PMC2446680.

Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R. Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 2010 Mar 9;20(5):441-5. doi: 10.1016/j.cub.2009.12.058. Epub 2010 Feb 25. PMID: 20188560.

Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.


  • Locus:
  • ODA2 [DHC15], TPG1 [FAP267]
  • Chromosome:
  • 11, 17

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124 32M

Origin: CRISPR mediated endogenous c-terminal mNeonGreen fusion knock-in

This strain has unusually bright fluorescent cilia which can be seen even in a conventional widefield fluorescent microscope. Paromomycin resistant.


Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.


  • Locus:
  • FMG1B (Cre09.g392867_4532)
  • Chromosome:
  • 9

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124 32M

Origin: CRISPR mediated endogenous n-terminal mNeonGreen with introns + c-terminal mScarlet-I knock-in

Proof of concept strain for serially tagging the same gene on both termini. IFT content in this strain is reduced in quantity but present. Resistant to Paromomycin and Blasticidin S.


Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.


  • Locus:
  • FT46 (Cre05.g241637_4532)
  • Chromosome:
  • 5

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124 32M

Origin: Co-targeted CRISPR mediated endogenous c-terminal mScarletI + DRC4::AphVIII insertion

Proof of concept strain for cotargeted genome editing with the possibility of crossing out the resistance marker in a second step. See CC-6015 for crossed out non-resistance IFT46-mScarletI progeny. Resistant to Paromomycin.


Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.


  • Locus:
  • IFT46 (Cre05.g241637_4532), DRC4 (Cre05.g241637_4532)
  • Chromosome:
  • 5, 5

From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023

Background: CC-124/CC-125

Origin: Back-cross of CC-6014 to CC-125 to restore wild-type DRC4 function and remove the AphVIII resistance marker previously used for selection

Proof of concept strain for cotargeted genome editing with the possibility of crossing out the resistance marker. See CC-6014 for original CRISPR strain with disrupted DRC4 gene. No antibiotic resistance present.


Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.


  • Locus:
  • IFT46 (Cre05.g241637_4532)
  • Chromosome:
  • 5