Strains
From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023
Phenotype: a truncation of the motor domain of outer arm dynein heavy chain γHC; lacks inner arm dynein I1/f; tends to form palmelloid cells
This mutant was obtained by crossing oda2-t (strain 12c, CC-6009) and ida1 (CC-2665). It is designated as strain 12c-1b in King lab.
Kamiya R, Kurimoto E, Muto E. Two types of Chlamydomonas flagellar mutants missing different components of inner-arm dynein. J Cell Biol. 1991 Feb;112(3):441-7. doi: 10.1083/jcb.112.3.441. PMID: 1825085; PMCID: PMC2288841.
Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008 Jul;7(7):1136-45. doi: 10.1128/EC.00102-08. Epub 2008 May 16. PMID: 18487347; PMCID: PMC2446680.
Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.
CC-6011 oda2-t tpg1 [#2]
$30.00
$30.00
From Miho Sakato-Antoku, Stephen M King lab, University of Connecticut Health, May 2023
Phenotype: a truncation of the motor domain of outer arm dynein heavy chain γHC; reduced axonemal tubulin polyglutamylation; mixed population of immotile cells and cells showing intermittent beating or swimming
This mutant was obtained by crossing oda2-t (strain 12c, CC-6009) and tpg1 (CC-5245).
Liu Z, Takazaki H, Nakazawa Y, Sakato M, Yagi T, Yasunaga T, King SM, Kamiya R. Partially functional outer-arm dynein in a novel Chlamydomonas mutant expressing a truncated gamma heavy chain. Eukaryot Cell. 2008 Jul;7(7):1136-45. doi: 10.1128/EC.00102-08. Epub 2008 May 16. PMID: 18487347; PMCID: PMC2446680.
Kubo T, Yanagisawa HA, Yagi T, Hirono M, Kamiya R. Tubulin polyglutamylation regulates axonemal motility by modulating activities of inner-arm dyneins. Curr Biol. 2010 Mar 9;20(5):441-5. doi: 10.1016/j.cub.2009.12.058. Epub 2010 Feb 25. PMID: 20188560.
Sakato-Antoku M, King SM. Outer-arm dynein light chain LC1 is required for normal motor assembly kinetics, ciliary stability, and motility. Mol Biol Cell. 2023 Jun 1;34(7):ar75. doi: 10.1091/mbc.E23-03-0104. Epub 2023 May 3. PMID: 37133971; PMCID: PMC10295483.
CC-6012 FMG1B-mNeonGreen
$30.00
$30.00
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124 32M
Origin: CRISPR mediated endogenous c-terminal mNeonGreen fusion knock-in
This strain has unusually bright fluorescent cilia which can be seen even in a conventional widefield fluorescent microscope. Paromomycin resistant.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124 32M
Origin: CRISPR mediated endogenous n-terminal mNeonGreen with introns + c-terminal mScarlet-I knock-in
Proof of concept strain for serially tagging the same gene on both termini. IFT content in this strain is reduced in quantity but present. Resistant to Paromomycin and Blasticidin S.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124 32M
Origin: Co-targeted CRISPR mediated endogenous c-terminal mScarletI + DRC4::AphVIII insertion
Proof of concept strain for cotargeted genome editing with the possibility of crossing out the resistance marker in a second step. See CC-6015 for crossed out non-resistance IFT46-mScarletI progeny. Resistant to Paromomycin.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124/CC-125
Origin: Back-cross of CC-6014 to CC-125 to restore wild-type DRC4 function and remove the AphVIII resistance marker previously used for selection
Proof of concept strain for cotargeted genome editing with the possibility of crossing out the resistance marker. See CC-6014 for original CRISPR strain with disrupted DRC4 gene. No antibiotic resistance present.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124 32M
Origin: Co-targeted CRISPR mediated endogenous n-terminal mNeonGreen with introns + DRC4::AphVIII insertion
Proof of concept strain for cotargeted genome editing with region replacement by multiple guide RNAs. The expression of fluorescent tubulin is visible in a confocal microscope, but very expression is unusually low. Resistant to Paromomycin.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
CC-6017 FAP256-mNeonGreen
$30.00
$30.00
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124 32M
Origin: CRISPR mediated endogenous c-terminal mNeonGreen fusion
This strain has bright fluorescent ciliary tips as well as zones of fluorescence clost to the ciliary base. Resistant to Nourseothricin.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-4375
Origin: Insertional rescue of IFT46::NIT
Red fluorescent label for IFT, but less bright than CC-6014/CC-6015. Resistant to Paromomycin.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Adrian Nievergelt, Max-Planck-Institute for Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany, August 2023
Background: CC-124 32M/CC-4375
Origin: Cross between CC-6017 and CC-6018
This strain has bright green fluorescent ciliary tips as well as zones of green fluorescence clost to the ciliary base and red fluorescent IFT. Resistant to Nourseothricin and Paromomycin.
Nievergelt AP, Diener DR, Bogdanova A, Brown T, Pigino G. Efficient precision editing of endogenous Chlamydomonas reinhardtii genes with CRISPR-Cas. Cell Rep Methods. 2023 Aug 22;3(8):100562. doi: 10.1016/j.crmeth.2023.100562. PMID: 37671018; PMCID: PMC10475843.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of RBCS2 (Cre02.g120150) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to hygromycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of RPE1 (Cre12.g511900) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of PRK1 (Cre12.g554800) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of EPYC1 (Cre10.g436550) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of RBCS2 (Cre02.g120150) as a fusion protein with a C-terminal TurboID and mCherry tag using the PSAD promoter/terminator pair. The strain carries resistance to hygromycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of RPE1 (Cre12.g511900) as a fusion protein with a C-terminal TurboID and mCherry tag using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of PRK1 (Cre12.g554800) as a fusion protein with a C-terminal TurboID and mCherry using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of EPYC1 (Cre10.g436550) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of the epyc1 mutant (https://doi.org/10.1073/pnas.1522866113) using a plasmid which drives expression of RBCS2 (Cre02.g120150) as a fusion protein with a C-terminal TurboID and 3xHA tag using the PSAD promoter/terminator pair. The strain carries resistance to both hygromycin and paromomycin resistance.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of RBCS2 (Cre02.g120150) as a fusion protein with a C-terminal APEX2 and 3xFlag tag using the PSAD promoter/terminator pair. The strain carries resistance to hygromycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of STR16 (Cre13.g573250) as a fusion protein with a C-terminal mScarlet-I and 3xFlag tag using its native promoter. The strain carries resistance to hygromycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of STR18 (Cre16.g663150) as a fusion protein with a C-terminal Venus and 3xFlag tag using its native promoter. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of protein encoded by Cre09.g394510 as a fusion protein with a C-terminal Venus and 3xFlag tag using its native promoter. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of ABCF6 (Cre06.g271850) as a fusion protein with a C-terminal Venus and 3xFlag tag using its native promoter. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of protein encoded by Cre03.g172700 as a fusion protein with a C-terminal Venus and 3xFlag tag using its native promoter. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of SMC7 (Cre17.g720450) as a fusion protein with a C-terminal Venus and 3xFlag tag using its native promoter. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Chun Sing Lau, Mackinder lab, University of York, July 2023
This strain was obtained by transformation of CC-4533 using a plasmid which drives expression of protein encoded by Cre02.g093650 as a fusion protein with a C-terminal Venus and 3xFlag tag using its native promoter. The strain carries resistance to paromomycin.
Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM. A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. Plant Cell. 2023 Sep 1;35(9):3260-3279. doi: 10.1093/plcell/koad131. PMID: 37195994; PMCID: PMC10473203.
From Olga Baidukova, Peter Hegemann lab, Humboldt University-Berlin, July 2023
From Olga Baidukova, Peter Hegemann lab, Humboldt University-Berlin, July 2023
From Olga Baidukova, Peter Hegemann lab, Humboldt University-Berlin, July 2023